抗Human/Mouse/Rat FoxP3 Alexa Fluor 647抗体(Anti-Human/Mouse/Rat FoxP3 Alexa Fluor 647 antibody)
掲載日情報：2018/11/26 現在Webページ番号：178173
Human/Mouse/Rat FoxP3 Alexa Fluor 647に対する抗体(Anti-Human/Mouse/Rat FoxP3 Alexa Fluor 647 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年06月07日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
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Anti-Human/Mouse/Rat FoxP3 Alexa Fluor 647 MAb (Clone 376209) |
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0 | |||
[在庫・価格 ：2024年06月07日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human/Mouse/Rat FoxP3 Alexa Fluor 647 MAb (Clone 376209)
文献数： 0
- 商品コード：IC8970R-025
- メーカー：RSD
- 包装：25tests
- 価格：¥42,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human, Mouse, Rat |
|---|---|
| Label | Alexa Fluor 647 |
| Immunogen | E. coli-derived recombinant human FoxP3Gln105-Lys200Accession # Q9BZS1 |
| Source | Recombinant Monoclonal Mouse IgG1 Clone # 376209 |
| Purification | Protein A or G purified from cell culture supernatant |
| Specificity | Detects human FoxP3 in direct ELISAs. Detects human, mouse, and rat FoxP3 in flow cytometry. |
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Applications and Data
| Recommended Concentration | Sample | |
| Intracellular Staining by Flow Cytometry | 5 µL/106 cells | See below |
| Intracellular Staining by Flow Cytometry | |
|---|---|
 | Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8970R) and Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8970R) and Mouse Anti-Human CD25/IL-2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8970R) and Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB554G). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8970R) and Rat Anti-Mouse CD25/IL-2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of FoxP3 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8970R) and Anti-Rat CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
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Related Product & Information
| Long Name | Forkhead Box P3 |
|---|---|
| Background | FoxP3 |
| background_content | Background: FoxP3 Human FoxP3 is a 431 amino acid (aa), 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three alternative splicing events have been described in human. The first shows a deletion of aa 72-106, a second exhibits a deletion of aa 246-272, and a third records a 60 aa insertion after Lys382. These events are likely not to be mutually exclusive. The first isoform (plus the full-length form) occurs in CD4+CD25+ T cells, while the less frequent second isoform appears in CD8+ Tregs. The mouse FoxP3 gene does not appear to be spliced. In addition, FoxP3 is found only in Tregs in mouse, while human FoxP3 appears in both Tregs and CD4+ and CD8+ conventional T cells.Both mouse and human FoxP3 undergo proteolytic processing, after Arg51 and Arg417 in human, and at equivalent sites in mouse. Cleavage at the N-terminus generate a 41 kDa isoform, whereas C-terminal cleavage generates an isoform indistinguishable from the full-length isoform (47 kDa) in SDS-PAGE.FoxP3 can act as a transcriptional repressor (for IL-2) via an interaction with HDAC7. By contrast, it can also act as a transcriptional activator (for GITRL andCTLA-4) through an interaction with RUNX7. Notably, for both human and mouse, full-length 47 kDa FoxP3 will induce high levels of CTLA-4 on expressing cells. However, when the C-terminally truncated isoform (at Arg417) is considered, IL-10 promoting activity is enhanced in mouse, while suppressive activity is lost in human. Over aa 105-200, human FoxP3 shares 83% and 84% aa sequence identity with rat and mouse FoxP3, respectively. |
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