抗Human ULBP-1抗体(Anti-Human ULBP-1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	BaF3 mouse pro-B cell line transfected with human ULBP-1
Source	Monoclonal Mouse IgG2A Clone # 170818
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 5-15 ng/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human ULBP-1 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) coated at 2 µg/mL (100 µL/well). At 300 ng/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of ULBP‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP‑1 Monoclonal Antibody (Catalog # MAB1380, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

目次に戻る

Related Product & Information

Long Name	UL16 Binding Protein-1
Entrez Gene IDs	80329 (Human); 77777 (Mouse)
Background	ULBP-1
background_content	Background: ULBP-1 ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

目次に戻る

Citations

1. Purine nucleotide metabolism regulates expression of the human immune ligand MICA
   Authors: MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha
   J. Biol. Chem., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells
   Authors: C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann
   Cell Death Dis, 2017;8(8):e2973.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model
   Authors: NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz
   JCI Insight, 2017;2(13):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
   Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
   Oncotarget, 2017;8(19):31959-31976.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells
   Authors: X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh
   Neoplasia, 2017;19(6):471-482.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
   Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
   Sci Rep, 2017;7(1):8661.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
   Sci Rep, 2016;6(0):32470.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
   Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
   Eur J Immunol, 2015;45(8):2324-34.
   Species: Human
   Sample Type: Plasma
   Application: IP


9. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
   Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
   BMC Cancer, 2015;15(0):351.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
    Authors: Kim, Ga Rim, Ha, Ga-Hee, Bae, Jae-Ho, Oh, Sae-Ock, Kim, Sun-Hee, Kang, Chi-Dug
    Oncol Lett, 2015;9(4):1641-1646.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

目次に戻る