抗Human ULBP-2/5/6抗体(Anti-Human ULBP-2/5/6 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	BaF3 mouse pro-B cell line transfected with human ULBP-2Accession # Q9BZM5
Source	Monoclonal Mouse IgG2A Clone # 165903
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.03 - 0.1 μg/mL of this antibody will block 50% of the binding of 25 ng/mL of biotinylated rhULBP-2/Fc to immobilized rhNKG2D/Fc chimera coated at 2 μg/mL (100 μL/well). At 1 μg/mL, this antibody will achieve 85% blocking in the assay. This antibody will block >70% of the binding of either Recombinant Human ULBP-5 Fc Chimera or Recombinant ULBP-6 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

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Related Product & Information

Long Name	UL16 Binding Protein-2/5/6
Background	ULBP-2/5/6
background_content	Background: ULBP-2/5/6 ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

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Citations

1. Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma
   Authors: S Weil, S Memmer, A Lechner, V Huppert, A Giannattas, T Becker, A Müller-Run, K Lampe, D Beutner, A Quaas, R Schubert, E Herrmann, A Steinle, U Koehl, L Walter, MS von Bergwe, J Koch
   Front Immunol, 2017;8(0):387.
   Application: ELISA detection


2. Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model
   Authors: NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz
   JCI Insight, 2017;2(13):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Purine nucleotide metabolism regulates expression of the human immune ligand MICA
   Authors: MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha
   J. Biol. Chem., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
   Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
   Sci Rep, 2017;7(1):8661.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells
   Authors: X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh
   Neoplasia, 2017;19(6):471-482.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells
   Authors: C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann
   Cell Death Dis, 2017;8(8):e2973.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
   Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
   Oncotarget, 2017;8(19):31959-31976.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
   Sci Rep, 2016;6(0):32470.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
   Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
   BMC Cancer, 2015;15(0):351.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
    Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
    Eur J Immunol, 2015;45(8):2324-34.
    Species: Human
    Sample Type: Plasma
    Application: IP

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