抗Mouse GM-CSF R alpha抗体(Anti-Mouse GM-CSF R alpha antibody)
掲載日情報：2018/11/26 現在Webページ番号：177414
Mouse GM-CSF R alphaに対する抗体(Anti-Mouse GM-CSF R alpha )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Mouse GM-CSF R alpha MAb (Clone 698423) |
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Anti-Mouse GM-CSF R alpha MAb (Clone 698423) |
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Anti-Mouse GM-CSF R alpha MAb (Clone 698423) |
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[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Mouse GM-CSF R alpha MAb (Clone 698423)
文献数： 0
- 商品コード：MAB6130-100
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Mouse GM-CSF R alpha MAb (Clone 698423)
文献数： 0
- 商品コード：MAB6130-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Mouse GM-CSF R alpha MAb (Clone 698423)
文献数： 0
- 商品コード：MAB6130-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha Leu30-Pro327Accession # Q00941 |
| Source | Monoclonal Rat IgG2A Clone # 698423 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha orrhGM-CSF R beta is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Neutralization | Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J. N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 0.1 ng/mL Recombinant Mouse GM‑CSF. | |
| Neutralization | |
|---|---|
 | Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF R alpha Antibody. Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GM‑CSF R alpha Monoclonal Antibody (Catalog # MAB6130). The ND50 is typically0.02-0.12 µg/mL. |
| Flow Cytometry | |
 | Detection of GM-CSF R alpha in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM‑CSF R alpha Monoclonal Antibody (Catalog # MAB6130, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). |
| Immunocytochemistry | |
 | GM‑CSF R alpha in J774A.1 Mouse Cell Line. GM‑CSF R alpha was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse GM‑CSF R alpha Monoclonal Antibody (Catalog # MAB6130) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Related Product & Information
| Long Name | Granulocyte Macrophage Growth Factor Receptor alpha |
|---|---|
| Entrez Gene IDs | 1438 (Human); 12982 (Mouse) |
| Background | GM-CSF R alpha |
| background_content | Background: GM-CSF R alpha Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Coincidental null mutation of Csf2r? in a colony of PI3K?-/- mice causes alveolar macrophage deficiency and fatal respiratory viral infection
J Leukoc Biol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Flow - Essential Role of mTORC1 in Self-Renewal of Murine Alveolar Macrophages
Authors: Xiao-Ping Zhong
J. Immunol., 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Flow - Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
Authors: Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F
J Exp Med, 2014;211(6):1243-56.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
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