抗Human PDGF R alpha抗体(Anti-Human PDGF R alpha antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha
Source	Monoclonal Mouse IgG1 Clone # 35248
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PDGF R beta or recombinant mouse PDGF R alpha is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human PDGF R alpha (Catalog # 322-PR) under non-reducing conditions only
Immunohistochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS‑1 human fetal skin fibroblast cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Neutralization
	Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the WS‑1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF R alpha Monoclonal Antibody (Catalog # MAB322). The ND50 is typically 2‑10 µg/mL.
Immunohistochemistry
	PDGF R alpha in Human Breast Cancer Tissue. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PDGF R alpha Monoclonal Antibody (Catalog # MAB322) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs	5156 (Human); 18595 (Mouse)
Background	PDGF R alpha
background_content	Background: PDGF R alpha PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

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Citations

1. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
   Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
   PLoS Pathog., 2017;13(4):e1006281.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Structural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recovery
   Authors: JM Yang, HS Lee, JH Seo, JH Park, IH Gelman, EH Lo, KW Kim
   Sci Rep, 2017;7(0):42723.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IF


3. Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
   Authors: Prignano F, Campolmi P, Bonan P
   Dermatol Ther, 2009;22(0):S8-15.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
   Authors: Li R, Maminishkis A, Wang FE, Miller SS
   Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
   Authors: Ball SG, Shuttleworth CA, Kielty CM
   J. Cell Biol., 2007;177(3):489-500.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
   Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
   J. Neurosci., 2006;26(47):12174-85.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions.
   Authors: Wilson HC, Scolding NJ, Raine CS
   J. Neuroimmunol., 2006;176(1):162-73.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
   Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
   Blood, 2006;107(5):1896-902.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
   Authors: Kubler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
   Prostate, 2005;63(4):385-94.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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