抗Human CCL3/MIP-1 alpha抗体(Anti-Human CCL3/MIP-1 alpha antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92Accession # P10147
Source	Monoclonal Mouse IgG1 Clone # 93321
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCL3/MIP-1 alpha in direct ELISAs and Western blots. In direct ELISAs, greater than 75% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is observed while approximately 30% cross-reactivity with recombinant mouse (rm) CCL3, rmCCL4/MIP-1 beta, and rhCCL14 is observed. No cross-reactivity with rhCCL1, 2, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, rmCCL1, 2, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) under non-reducing conditions only
Immunocytochemistry	8-25 µg/mL	Immersion fixed human peripheral blood mononuclear cells treated with PHA
Neutralization	Measured by its ability to neutralize CCL3/MIP-1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.075-0.375 µg/mL in the presence of 10 ng/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.

Neutralization

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Human CCL3/CCL4 Antibody. Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL3/CCL4 Monoclonal Antibody (Catalog # MAB270). The ND50 is typically 0.075‑0.375 µg/mL.

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Related Product & Information

Long Name	CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive
Background	CCL3/CCL4
background_content	Background: CCL3/CCL4 The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP‑1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP‑1 alpha. Mature MIP-1 alpha and MIP‑1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP‑1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP‑1 alpha and MIP‑1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP‑1 alpha has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP‑1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP‑1 alpha has been identified as CCR1 and CCR5.

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Citations

1. Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
   Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
   PLoS Pathog., 2018;14(2):e1006888.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Elevated ACKR2 expression is a common feature of inflammatory arthropathies
   Authors: HM Baldwin, MD Singh, V Codullo, V King, H Wilson, I McInnes, GJ Graham
   Rheumatology (Oxford), 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Macrophage inflammatory protein derivative ECI301 enhances the alarmin-associated abscopal benefits of tumor radiotherapy.
   Authors: Kanegasaki S, Matsushima K, Shiraishi K, Nakagawa K, Tsuchiya T
   Cancer Res, 2014;74(18):5070-8.
   Species: Mouse
   Sample Type: Beads
   Application: Co-immunoprecipitation


4. A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-alpha and MIP1-beta, a lack of IL-17, and a Th1-bias in males.
   Authors: Snyder-Cappione JE, Tincati C, Eccles-James IG, Cappione AJ, Ndhlovu LC, Koth LL, Nixon DF
   PLoS ONE, 2010;5(11):e15412.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISpot Development


5. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
   Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
   Cancer Res., 2009;69(15):6249-55.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Induction of host chemotactic response by Encephalitozoon spp.
   Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
   Infect. Immun., 2007;75(4):1619-25.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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