抗Human BDNF抗体(Anti-Human BDNF antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNFArg128-Arg247Accession # P23560
Source	Monoclonal Mouse IgG1 Clone # 35928
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human BDNF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross-reactivity with recombinant human (rh) beta -NGF and rr beta -NGF.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human BDNF (Catalog # 248-BD)
Immunohistochemistry	8-25 µg/mL	See below

Immunohistochemistry

BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Human BDNF Monoclonal Antibody (Catalog # MAB248) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

目次に戻る

Related Product & Information

Background

BDNF

background_content

Background: BDNF Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

目次に戻る

Citations

1. CD44v3+/CD24- cells possess cancer stem cell-like properties in human oral squamous cell carcinoma.
   Authors: Todoroki K, Ogasawara S, Akiba J, Nakayama M, Naito Y, Seki N, Kusukawa J, Yano H
   Int J Oncol, 2015;48(1):99-109.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


2. Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival.
   Authors: Vanhecke E, Adriaenssens E, Verbeke S, Meignan S, Germain E, Berteaux N, Nurcombe V, Le Bourhis X, Hondermarck H
   Clin. Cancer Res., 2011;17(7):1741-52.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Decreased BDNF levels are a major contributor to the embryonic phenotype of huntingtin knockdown zebrafish.
   Authors: Diekmann H, Anichtchik O, Fleming A, Futter M, Goldsmith P, Roach A, Rubinsztein DC
   J. Neurosci., 2009;29(5):1343-9.
   Species: Zebrafish
   Sample Type: Cell Lysates
   Application: WB


4. Brain-derived neurotrophic factor (BDNF) and TrkB in the piglet brainstem after post-natal nicotine and intermittent hypercapnic hypoxia.
   Authors: Tang S, Machaalani R, Waters KA
   Brain Res., 2008;1232(0):195-205.
   Species: Porcine
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
   Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
   Cancer Res., 2008;68(23):9996-10003.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
   Authors: Samah B, Porcheray F, Gras G
   Clin. Exp. Immunol., 2008;151(3):476-86.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
   Authors: Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
   J. Neuroimmunol., 2008;197(2):128-39.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Brain-derived neurotrophic factor mRNA and protein in the piglet brainstem and effects of Intermittent Hypercapnic Hypoxia.
   Authors: Peiris TS, Machaalani R, Waters KA
   Brain Res., 2004;1029(1):11-23.
   Species: Porcine
   Sample Type: Tissue Homogenates
   Application: WB

目次に戻る