抗Human EGF抗体(Anti-Human EGF antibody)
掲載日情報：2018/11/26 現在Webページ番号：177240
Human EGFに対する抗体(Anti-Human EGF )です。
※ 本製品は研究用です。研究用以外には使用できません。
カートに商品を
追加しました。
追加しました。
価格
[在庫・価格 ：2025年11月03日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
|
Anti-Human EGF MAb (Clone 10825) |
|
11 | |||
|
Anti-Human EGF MAb (Clone 10825) |
|
11 | |||
|
Anti-Human EGF MAb (Clone 10825) |
|
11 | |||
[在庫・価格 ：2025年11月03日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human EGF MAb (Clone 10825)
文献数： 11
- 商品コード：MAB236-100
- メーカー：RSD
- 包装：100μg
- 価格：¥81,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human EGF MAb (Clone 10825)
文献数： 11
- 商品コード：MAB236-500
- メーカー：RSD
- 包装：500μg
- 価格：¥271,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human EGF MAb (Clone 10825)
文献数： 11
- 商品コード：MAB236-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥28,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
カートに商品を
追加しました。
追加しました。
Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human EGF |
| Source | Monoclonal Mouse IgG1 Clone # 10825 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed. |
カートに商品を
追加しました。
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Neutralization | Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF. | |
| Western Blot | |
|---|---|
 | Detection of Recombinant Human and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
| Immunohistochemistry | |
 | EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Neutralization | |
 | Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND50 is typically0.05‑0.1 µg/mL. |
カートに商品を
追加しました。
追加しました。
Related Product & Information
| Long Name | Epidermal Growth Factor |
|---|---|
| Entrez Gene IDs | 1950 (Human); 13645 (Mouse); 25313 (Rat) |
| Background | EGF |
| background_content | Background: EGF EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells. |
カートに商品を
追加しました。
追加しました。
Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells
Authors: S Müller-Deu, L Seefried, M Krug, F Jakob, R Ebert
Stem Cell Res, 2017;24(0):69-76.
Species: Human
Sample Type: Whole Cells
Application: Functional Assay - Expression and clinical value of EGFR in human meningiomas
Authors: MB Arnli, T Backer-Grø, B Ytterhus, US Granli, S Lydersen, S Gulati, SH Torp
PeerJ, 2017;5(0):e3140.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Case report of multiple pustules of the bilateral lower limbs caused by a granulocyte colony-stimulating factor-producing solid pseudopapillary tumour of the pancreas
Authors: Y Iwata
Br. J. Dermatol, 2016;0(0):.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
Cancer Res, 2014;74(10):2869-81.
Species: Human
Sample Type: Whole Cells
Application: Neut - Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010;7(0):13.
Species: Porcine
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - S100A11, an dual mediator for growth regulation of human keratinocytes.
Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Mol. Biol. Cell, 2007;19(1):78-85.
Species: Human
Sample Type: Cell Lysates
Application: WB - Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
J. Invest. Dermatol., 2006;126(9):2096-105.
Species: Human
Sample Type: Serum
Application: Neut - Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
J. Mol. Endocrinol., 2005;34(2):353-66.
Species: Human
Sample Type: Cell Lysates
Application: WB
カートに商品を
追加しました。
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。