抗His Tag抗体(Anti-His Tag antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Label	Unconjugated
Immunogen	His-tagged peptide
Source	Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	10 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunoaffinity Purification	Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.

Western Blot
	Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Simple Western
	Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of CHO Chinese hamster ovary cell line transfected with His-tagged human S1P4/EDG-6, loaded at 0.5 mg/mL. A specific band was detected for His-tagged S1P4/EDG-6 at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Histidine Tag
Background	His Tag
background_content	Background: His Tag Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

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Citations

1. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
   Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
   Immunity, 2017;46(5):804-817.e7.
   Species: Primate
   Sample Type: Plasma
   Application: ELISA Capture


2. Hypoxia favors the generation of human plasma cells
   Authors: M Schoenhals, M Jourdan, A Bruyer, A Kassambara, B Klein, J Moreaux
   Cell Cycle, 2017;0(0):1-14.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


3. Biophysical analysis of the dynamics of calmodulin interactions with neurogranin and Ca2+/calmodulin-dependent kinase II
   Authors: C Seeger, VO Talibov, UH Danielson
   J. Mol. Recognit., 2017;0(0):.
   Application: Bioassay


4. Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein
   Authors: JH Urban, MA Moosmeier, T Aumüller, M Thein, T Bosma, R Rink, K Groth, M Zulley, K Siegers, K Tissot, GN Moll, J Prassler
   Nat Commun, 2017;8(1):1500.
   Application: Bioassay


5. Structural basis of homo- and heterotrimerization of collagen I
   Authors: U Sharma, L Carrique, S Vadon-Le G, N Mariano, RN Georges, F Delolme, P Koivunen, J Myllyharju, C Moali, N Aghajari, DJ Hulmes
   Nat Commun, 2017;8(0):14671.
   Species: Human
   Sample Type: Protein
   Application: WB


6. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
   Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
   J Biol Chem, 2016;0(0):.
   Species: Human
   Sample Type: Complex Sample Type
   Application: Bioassay


7. Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
   Authors: Alexey A Lugovskoy
   MAbs, 2016;0(0):0.
   Application: Flow


8. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
   Sci Rep, 2016;6(0):36862.
   Species: Human
   Sample Type: Whole Cells
   Application: Cross-linking


9. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies
    PLoS Pathog, 2016;12(8):e1005767.
    Species: Guinea Pig
    Sample Type: Serum
    Application: ELISA Capture

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