抗Histone H3 (1B1-B2)抗体 | Anti-Histone H3 (1B1-B2) Antibody

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	Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Western blot image of Anti-Histone H3 antibody (clone 1B1-B2). Whole cell protein from HeLa (lane 1), NIH-3T3 (lane 2) and PC12 (lane 3) were separated by 4-15% SDS-PAGE and transfered to PVDF membrane. The membrane was probed with purified Anti-Histone H3 antibody at 2 ug/ml and detected with an HRP conjugated anti-mouse secondary using chemiluminescence.
	Immunohistochemistry-Paraffin: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.
	Flow Cytometry: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-36468. Jurkat cells stained with Alexa Fluor 647 conjugated Histone H3 antibody (red) or isotype control (blue). Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: Histone H3 Antibody (1B1-B2) [NBP2-36468] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Histone H3 (1B1-B2) NBP2-36468 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Flow (Intracellular): Histone H3 Antibody (1B1-B2) [NBP2-36468] - An intracellular stain was performed on HeLa cells with Histone H3 Antibody (1B1-B2) NBP2-36468PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
	Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.

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Background

Eukaryotic chromatin exists as nucleosomes, each of which is composed of an octamer of histone molecules, around which the DNA is wrapped. Each octamer contains two molecules each of histones H2A, H2B, H3 and H4. Histone proteins consist of a globular domain and a flexible N-terminal tail; the tail protrudes from the nucleosome and is the site of a huge diversity of post-translational modifications that support the maintenance of an active or inactive transcriptional state. Numerous modifications of histone H3 are known, including methylation or acetylation of arginine or lysine residues, and phosphorylation of serine or threonine residues; these modifications may promote or inhibit transcription. There are three variants of histone H3, known as H3.1, H3.2 and H3.3, each of which is encoded by a number of different genes. Although these variants share similar amino acid sequences, they differ in their expression pattern and post-translational modifications and perform distinct functions.

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