抗Histone H3 [Trimethyl Lys27]抗体 | Anti-Histone H3 [Trimethyl Lys27] Antibody

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	Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
	Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Immunofluorescence photomicrographs of frozen sections of mouse liver. Red: Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody Blue: Nuclear staining with Hoechst 33342.
	Immunohistochemistry Whole-Mount: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3K27me3 (trimethyl Lys27) antibody dilution: 1:100.
	Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control).
	Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at a dilution of 1:1000.
	Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Sample (30 ug of whole cell lysate) A: Jurkat, B: Raji, C: K562, D: THP-1, 7.5% SDS PAGE diluted at 1:500
	Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A (tri-Methyl K27) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
	Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
	Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraffin-embedded mouse brain Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500.
	Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraffin-embedded mouse duodenum Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500
	Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at 1:2500.

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

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