抗PolH, Rabbit-Poly抗体 | Anti-PolH, Rabbit-Poly Antibody

• 価格
• 特長
• 製品情報
• 使用例画像
• 文献情報

価格

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特長

Reactivity	Human
Applications	WB, IP
Host	Rabbit
Antibody Type	Polyclonal
Conjugate	Unconjugated
Format	Whole IgG

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製品情報

Immunogen	Between 690 and 713
100% Sequence Identity	Mouse
Gene ID	5429
Gene Name	polymerase (DNA) eta
Gene Symbol	POLH
Uniprot ID	Q9Y253
Protein Name	DNA polymerase eta

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使用例画像

Detection of human and mouse PolH by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolH antibody A301-231A (lot A301-231A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PolH antibody A301-231A (lot A301-231A-2) used for IP at 3 µg per reaction. PolH was also immunoprecipitated by rabbit anti-PolH antibody A301-230A. For blotting immunoprecipitated PolH, A301-231A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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文献情報

1. DNA repair factor RAD18 and DNA polymerase Polκ confer tolerance of oncogenic DNA replication stress.
   In The Journal of Cell Biology on 2 October 2017 by Yang, Y., Gao, Y., et al..
2. Predominant role of DNA polymerase eta and p53-dependent translesion synthesis in the survival of ultraviolet-irradiated human cells.
   In Nucleic Acids Research on 17 February 2017 by Lerner, L. K., Francisco, G., et al..
3. Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.
   In Nature Communications on 4 November 2016 by Despras, E., Sittewelle, M., et al..
4. Break-induced telomere synthesis underlies alternative telomere maintenance.
   In Nature on 3 November 2016 by Dilley, R. L., Verma, P., et al..
5. A neomorphic cancer cell-specific role of MAGE-A4 in trans-lesion synthesis.
   In Nature Communications on 5 July 2016 by Gao, Y., Mutter-Rottmayer, E., et al..
6. A non-catalytic role of DNA polymerase η in recruiting Rad18 and promoting PCNA monoubiquitination at stalled replication forks.
   In Nucleic Acids Research on 1 March 2013 by Durando, M., Tateishi, S., et al..
7. Cell cycle stage-specific roles of Rad18 in tolerance and repair of oxidative DNA damage.
   In Nucleic Acids Research on 1 February 2013 by Yang, Y., Durando, M., et al..
8. c-Jun N-terminal kinase-mediated Rad18 phosphorylation facilitates Polη recruitment to stalled replication forks.
   In Molecular Biology of the Cell on 1 May 2012 by Barkley, L. R., Palle, K., et al..
9. Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability.
   In The EMBO Journal on 15 February 2012 by Jones, M. J., Colnaghi, L., et al..
10. Phosphorylated Rad18 directs DNA polymerase η to sites of stalled replication.
    In The Journal of Cell Biology on 29 November 2010 by Day, T. A., Palle, K., et al..

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