抗MCM2, Rabbit-Poly抗体 | Anti-MCM2, Rabbit-Poly Antibody

• 価格
• 特長
• 製品情報
• 使用例画像
• 文献情報

価格

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特長

Reactivity	Human, Mouse
Applications	WB, IP, IHC
Host	Rabbit
Antibody Type	Polyclonal
Conjugate	Unconjugated
Format	Whole IgG

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製品情報

Immunogen	Between 1 and 50
Gene ID	4171
Gene Name	minichromosome maintenance complex component 2
Gene Symbol	MCM2
Uniprot ID	P49736
Protein Name	DNA replication licensing factor MCM2

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使用例画像

	Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody A300-191A (lot A300-191A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
	Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody A300-191A (lot A300-191A-2) used for IP at 3 µg per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody A300-094A. For blotting immunoprecipitated MCM2, A300-191A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
	Detection of human MCM2 by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 (Cat. No. A300-191A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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文献情報

1. MCM2-7-dependent cohesin loading during S phase promotes sister-chromatid cohesion.
   In eLife on 3 April 2018 by Zheng, G., Kanchwala, M., et al..
2. Identification and Characterization of MCM3 as a Kelch-like ECH-associated Protein 1 (KEAP1) Substrate.
   In The Journal of Biological Chemistry on 4 November 2016 by Mulvaney, K. M., Matson, J. P., et al..
3. Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases.
   In Biology Open on 15 October 2016 by Lu, F., Wu, X., et al..
4. A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves.
   In Scientific Reports on 23 August 2016 by Andersen, N. D., Srinivas, S., et al..
5. The potent Cdc7-Dbf4 (DDK) kinase inhibitor XL413 has limited activity in many cancer cell lines and discovery of potential new DDK inhibitor scaffolds.
   In PLoS ONE on 21 November 2014 by Sasi, N. K., Tiwari, K., et al..
6. Replication stress by Py-Im polyamides induces a non-canonical ATR-dependent checkpoint response.
   In Nucleic Acids Research on 1 October 2014 by Martínez, T. F., Phillips, J. W., et al..
7. Adaptive auto-regulation of androgen receptor provides a paradigm shifting rationale for bipolar androgen therapy (BAT) for castrate resistant human prostate cancer.
   In The Prostate on 1 October 2012 by Isaacs, J. T., D'Antonio, J. M., et al..
8. Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA.
   In Nucleic Acids Research on 1 July 2011 by Guéranger, Q., Kia, A., et al..
9. L3MBTL1 polycomb protein, a candidate tumor suppressor in del(20q12) myeloid disorders, is essential for genome stability.
   In Proceedings of the National Academy of Sciences of the United States of America on 28 December 2010 by Gurvich, N., Perna, F., et al..
10. The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription.
    In The Journal of Biological Chemistry on 15 May 2009 by Snyder, M., Huang, X. Y., et al..

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