抗Myosin抗体(Anti-Myosin, Heavy Chain, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Label	Unconjugated
Immunogen	Chicken pectoralis-derived Myosin
Source	Monoclonal Mouse IgG2B Clone # MF20
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	Stains, C.I., et al. (2012) Chem Biol. 19:210.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

Myosin Heavy Chain in C2C12 Mouse Cell Line. Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Myosin Heavy Chain

background_content

Background: Myosin Heavy Chain Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

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Citations

1. Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors
   Authors: F Sakai-Take, A Narita, S Masuda, T Wakamatsu, N Watanabe, T Nishiyama, K Nogami, M Blanc, S Takeda, Y Miyagoe-Su
   Sci Rep, 2018;8(1):6555.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice
   Authors: T Sasaki, A Kuboyama, M Mita, S Murata, M Shimizu, J Inoue, K Mori, R Sato
   J. Biol. Chem., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration
   Authors: M Koide, Y Hagiwara, M Tsuchiya, M Kanzaki, H Hatakeyama, Y Tanaka, T Minowa, T Takemura, A Ando, T Sekiguchi, Y Yabe, E Itoi
   Tohoku J. Exp. Med., 2018;244(1):15-24.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients
   Authors: M Wattin, L Gaweda, P Muller, M Baritaud, C Scholtes, C Lozano, K Gieseler, C Kretz-Remy
   Int J Mol Sci, 2018;19(1):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation
   Authors: DS Han, WS Yang, TW Kao
   Int J Med Sci, 2017;14(5):434-443.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy
   Authors: H Lee, SJ Lee, GU Bae, NI Baek, JH Ryu
   Int J Mol Sci, 2017;18(12):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


7. NF?B signaling in alveolar rhabdomyosarcoma
   Authors: MM Cleary, A Mansoor, T Settelmeye, Y Ijiri, KJ Ladner, MN Svalina, BP Rubin, DC Guttridge, C Keller
   Dis Model Mech, 2017;10(9):1109-1115.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
   Authors: G Zhang, Z Liu, H Ding, Y Zhou, HA Doan, KWT Sin, ZJ Zhu, R Flores, Y Wen, X Gong, Q Liu, YP Li
   Nat Commun, 2017;8(1):589.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


9. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor
   Authors: S Kokabu, C Nakatomi, T Matsubara, Y Ono, WN Addison, JW Lowery, M Urata, AM Hudnall, S Hitomi, M Nakatomi, T Sato, K Osawa, T Yoda, V Rosen, E Jimi
   J. Biol. Chem., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


10. R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways
    Authors: F Lacour, E Vezin, CF Bentzinger, MC Sincennes, L Giordani, A Ferry, R Mitchell, K Patel, MA Rudnicki, MC Chaboissie, AA Chassot, F Le Grand
    Cell Rep, 2017;18(10):2320-2330.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

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