HMGB1, CF 組換え体(リコンビナント)タンパク質 | Recombinant Human HMGB1, CF
掲載日情報:2015/07/09 現在Webページ番号:160001
R&D Systems社製の高品質なHMGB1, CFの組換え体タンパク質(Recombinant Human HMGB1, CF)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年08月18日 20時55分現在]
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[在庫・価格 :2025年08月18日 20時55分現在]
Recombinant Human HMGB1, CF
文献数: 10
- 商品コード:1690-HMB-050
- メーカー:RSD
- 包装:50μg
- 価格:¥91,000
- 在庫:無(発注済)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 純度:>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by CoomassieR Blue Staining.,由来動物:Human,M.W.:25 kDa,Genbank:3146,エンドトキシンレベル:<0.10 EU per 1 μg of the protein by the LAL method.,産生:NS0 別名:Amphoterin Genbank No: 3146 Protein Accession No: P09429 |
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保存条件 | -20℃ | 法規備考 | |
掲載カタログ |
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製品記事 | 抗HMGB1 / HMG-1抗体 |
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関連記事 | R&D Systems製品 価格改定(値下げ)のご案内 |
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バルク注文に関して
※バルク包装品も承ります。お気軽にお問合せ下さい。
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CF(Carrier-Free)とは?
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Source
- Mouse myeloma cell line, NS0-derived, Met1-Glu215
- Accession
- P09429
- Purity
- >95%, by SDS-PAGE with silver staining.
- Predicted Molecular Mass
- 25 kDa
- SDS-PAGE
- 28-34 kDa, reducing conditions
- Activity
- Measured by its ability to induce TNF-alphasecretion by RAW 264.7 mouse monocyte/macrophage cells.The ED50 for this effect is typically 2.5-12.5 µg/mL.Measured in a competitive binding assay. When Recombinant Human RAGE Fc Chimera (Catalog # 1145-RG) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human HMGB1/HMG-1 inhibits 50% binding of biotinylated recombinant human HMGB1 (0.25 µg/mL) at the concentration range of 0.35-1.4 µg/mL.
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Additional Information
- Molecule Information
- HMGB1/HMG-1
- Aliases
- HMG-1
- Entrez Gene IDs
- 3146 (Human)
- Background
- HMGB1
- High mobility Group Box 1 (HMGB1) protein is a highly conserved, abundant protein present in the nuclei and cytoplasm of almost all cells. It participates in maintaining nucleosome structure, regulating steroid hormone receptor action, and in the mediation of gene transcription. HMGB1 is also secreted into the extracellular fluid and acts as a proinflammatory cytokine.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Cancer usurps skeletal muscle as an energy repository.
Chihara , Yoshitom, Eto , Sachise, Kato , Yumiko, Kobayashi , Tsuyoshi, Kuniyasu , Hiroki, Luo , Yi, Miyano , Hiroshi, Ohmori , Hitoshi, Sasahira , Tomonori, Sasaki , Takamits, Shimbo , Kazutaka, Yoneda , Junya
Cancer Res 2014 74:330-40
Species:
Protein Application: Bioassay
S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.
Clin. Cancer Res. 2012 18:4356-64
The persistent release of HMGB1 contributes to tactile hyperalgesia in a rodent model of neuropathic pain.
J Neuroinflammation 2012 9:180
Species: Rat
Protein Application: Bioassay
Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury.
Chen , Jianlin, Lu , Christop, Matzuk , Martin M, Zhou , Xin J
Kidney Int 2012 82:1195-207
Species: Human
Protein Application: Bioassay
Effects of high mobility group box protein-1, interleukin-1beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures.
Ekman S, Eloranta ML, Ley C, Lindahl A, Nilton A, Skioldebrand E, Svala E
Connect. Tissue Res. 2011 52:290-300
Species: Equine
Protein Application: Bioassay
TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding.
Hibino T, Huh NH, Kataoka K, Motoyama A, Murata H, Ono T, Sakaguchi M, Sakaguchi Y, Yamamoto K
PLoS ONE 2011 6:e23132
Species: Human
Protein Application: Bioassay
Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
Argyle B, Hoidal JR, Kennedy TP, MacArthur RB, Prechel M, Prestwich GD, Rao NV, Reynolds PR, Walenga JM, Walters BB, Xu X
Am. J. Physiol., Cell Physiol. 2010 299:C97-110
Species: Human
Protein Application: In Vivo
Complement C3a, CpG oligos, and DNA/C3a complex stimulate IFN-alpha production in a receptor for advanced glycation end product-dependent manner.
Cunningham KM, Fitz LJ, Greco RM, Kuai J, Li X, Lin LL, Nocka KH, Paulsen JE, Pittman DD, Ruan BH, Tan XY, Winkler AR, Winkler DG, Wright JF
J. Immunol. 2010 185:4213-22
Species: Human
Protein Application: Binding Assay
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
Chan CC, Chen DQ, Guan XY, Huang JD, Lu L, Ng F, Poon VK, Watt RM, Yuen KY, Zheng BJ, Zhou J
J. Hepatol. 2009 51:322-32
Species: N/A
Protein Application: Western Blot Control
Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
Andre F, Apetoh L, Aymeric L, Civas A, Genin P, Ghiringhelli F, Haynes NM, Kanellopoulos J, Kroemer G, Lidereau R, Ma Y, McLaughlin NM, Mignot G, Ortiz C, Panaretakis T, Perfettini JL, Ryffel B, Schlemmer F, Smyth MJ, Tasdemir E, Tesniere A, Tschopp J, Uhl M, Ullrich E, Vermaelen K, Zitvogel L
Nat. Med. 2009 15:1170-8
Species: Mouse
Protein Application: Bioassay
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