抗Mouse IL-15 R alpha Allophycocyanin抗体(Anti-Mouse IL-15 R alpha Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha Gly33-Lys205Accession # Q60819
Source	Monoclonal Rat IgG2B Clone # 888220
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-15 R alpha in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of IL‑15 R alpha in C57BL/6 Mouse Bone Marrow Cells by Flow Cytometry. C57BL/6 mouse bone marrow cells treated with 20 ng/mL Recombinant Mouse GM‑CSF (Catalog # 415-ML) for 5 days, 1 μg/mL LPS on day 6, and harvested on day 7 were stained with Rat Anti-Mouse IL‑15 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB5511A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Interleukin 15 Receptor alpha
Background	IL-15 R alpha
background_content	Background: IL-15 R alpha Interleukin 15 Receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, and through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha ‑mediated apoptosis in fibroblasts by competing with TNFRI for TRAF2 binding.

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Citations

1. Reprogramming the lung microenvironment by inhaled immunotherapy fosters immune destruction of tumor
   Authors: Valentino Le Noci
   Oncoimmunology, 2016;5(11):e1234571.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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