抗Mouse/Rat CD31/PECAM-1 Allophycocyanin抗体(Anti-Mouse/Rat CD31/PECAM-1 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1Glu18-Lys590Accession # Q08481
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

CD31/PECAM-1

background_content

Background: CD31/PECAM-1 PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

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Citations

1. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
   Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
   Nat. Cell Biol, 2017;19(3):189-201.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. MiR-497?195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1? activity
   Authors: M Yang, CJ Li, X Sun, Q Guo, Y Xiao, T Su, ML Tu, H Peng, Q Lu, Q Liu, HB He, TJ Jiang, MX Lei, M Wan, X Cao, XH Luo
   Nat Commun, 2017;8(0):16003.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Age-dependent modulation of vascular niches for haematopoietic stem cells
   Authors: AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams
   Nature, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Age-dependent modulation of vascular niches for haematopoietic stem cells
   Authors: AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams
   Nature, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
   Authors: Kusumbe A, Ramasamy S, Adams R
   Nature, 2014;507(7492):323-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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