抗5-Methylcytosine / 5-mC [33D3]抗体 | Anti-5-Methylcytosine / 5-mC [33D3] antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	33D3
Isotype	IgG1
Application	ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, Immunoblotting, MeDIP

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
ELISA	Assay dependent
Immunoblotting	Assay dependent
MeDIP	Assay dependent

Not tested in other applications.

Specificity/Sensitivity	This antibody recognizes 5-Methylcytosine. Clone 33D3 is specific to the methylated base and has minimal reactivity to non-methylated cytidine or cytosine (Reynaudet al.1992).

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.01% Thiomersal
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Purification	Purified IgG
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wilson RL et al. Epigenetics 2019; 14 (7) : 660-671 Characterization of 5-methylcytosine and 5-hydroxymethylcytosine in human placenta cell types across gestation.	Application : IHC-P
Chen CH et al. Anim Biotechnol 2016; 27 (3) : 157-65 Germline Competent Pluripotent Mouse Stem Cells Generated by Plasmid Vectors.	Application : ICC/IF
Gertych A et al. Exp Cell Res 2010; 316 (19) : 3150-60 Measuring topology of low-intensity DNA methylation sites for high-throughput assessment of epigenetic drug-induced effects in cancer cells.	Application : ICC/IF
Celik S et al. PLoS One 2014; 9 (4):e92523 The exit of mouse embryonic fibroblasts from the cell-cycle changes the nature of solvent exposure of the 5'-methylcytosine epitope within chromatin.	Application : FACS

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