抗HIF1 alpha [GT10211]抗体 | Anti-HIF1 alpha [GT10211] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	HIF1 alpha antibody [GT10211] detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha stained by HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
	HIF1 alpha antibody [GT10211] detects HIF1A protein by western blot analysis.A. 30 μg HepG2 whole cell lysate/extract (untreated)B. 30 μg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr)7.5% SDS-PAGEHIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments.IP Sample: 1000 μg HepG2 whole cell lysate/extract A. 40 μg HepG2 whole cell lysate/extract from normoxiaB. 40 μg HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)C. Control with 2.5 μg of preimmune mouse IgG/HepG2 whole cell lysate/extract from normoxiaD. Control with 2.5 μg of preimmune mouse IgG/ HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)E. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1 alpha antibody (GTX628480)/HepG2 whole cell lysate/extract from normoxiaF. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1 alpha antibody (GTX628480)/HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)10% SDS-PAGE The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
	HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 500 μM CoCl2 treatment for 24hr) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500μM CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	GT10211
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:100-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW	93 kDa. ( Note )
Positive Control	HepG2 (hypoxia:1%O2 48hr)

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The immunogen used to generate this antibody corresponds to human HIF1 alpha
Purification	Affinity purified by Protein G.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78
Cellular Localization	Cytoplasm , Nucleus
Background	Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Database	・ Gene ID: 3091 HIF1A ・ UniProt: Q16665 HIF1A

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Sharma S et al. Genome Biol 2019; 20 (1) : 37 Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.	Application : WB Reactivity : Human
Masola V et al. Oncotarget 2018; 9 (90) : 36185-36201 Inhibition of heparanase protects against chronic kidney dysfunction following ischemia/reperfusion injury.	Application : WB Reactivity : Mouse
Matsumura-Matsuda E et al. Intern Med 2018; 57 (7) : 979-985 A Rare Coexistence of Pheochromocytoma and Parkinson's Disease With Diagnostic Challenges.	Application : IHC-P Reactivity : Human
Shih JW et al. Nat Commun 2017; 8 : 15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.	Application : WB Reactivity : Human
Berezowska S et al. Virchows Arch 2017; (Epub) Glycine decarboxylase and HIF-1α expression are negative prognostic factors in primary resected early-stage non-small cell lung cancer.	Application : IHC-P Reactivity : Human

目次に戻る