抗c-Myc [GT168]抗体 | Anti-c-Myc [GT168] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	c-Myc antibody [GT168] detects Myc protein at nucleus on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. c-Myc antibody [GT168] (GTX628459) dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
	Immunoprecipitation of c-Myc protein from HeLa nuclear extracts using 5 μg of c-Myc antibody [GT168] (GTX628459).Western blot analysis was performed using c-Myc antibody [GT168] (GTX628459).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
	c-Myc antibody [GT168] antibody (GTX628459) detects c-Myc protein by flow cytometry analysis. Sample: NT2D1 cell fixed in 4% paraformaldehyde for 15 min on ice. Black: Unlabelled sample was also used as a control. Red: c-Myc antibody [GT168] antibody (GTX628459) dilution: 1:50. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
	c-Myc antibody [GT168] detects Myc protein by Western blot analysis.A. 30 μg 293T whole cell lysate/extract10 % SDS-PAGEc-Myc antibody [GT168] (GTX628459) dilution: 1:500
	c-Myc antibody [GT168] detects C-MYC protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: c-Myc antibody [GT168] dilution: 1:50

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	GT168
Isotype	IgG1
Application	WB, IHC-P, FACS, IP
Reactivity	Human

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500

Not tested in other applications.

Calculated MW	49 kDa. ( Note )
Positive Control	293T

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The immunogen used to generate this antibody corresponds to human c-Myc
Purification	Affinity purified by Protein G.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc
Cellular Localization	Nucleus
Background	The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]
Database	・ Gene ID: 4609 MYC ・ UniProt: P01106 MYC

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wu TH et al. Int J Mol Sci 2019; 20 (23) Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells.	Application : WB Reactivity : Human
Yang CE et al. Bioresour Technol 2017; (Epub) Surface display of synthetic phytochelatins on Saccharomyces cerevisiae for enhanced ethanol production in heavy metal-contaminated substrates.
Yao-Yu H et al. Oncotarget 2016; (Epub) Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.	Application : WB Reactivity : Human

目次に戻る