抗DNMT1 [60B1220.1]抗体 | Anti-DNMT1 [60B1220.1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	ICC/IF analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Green : primary antibodyRed : TubulinBlue : DAPI Dilution : 1:10
	WB analysis of HeLa cell lysate using GTX48548 DNMT1 antibody [60B1220.1].
	WB analysis of 2102EP cells / human DNMT1 recombinant protein / NIH3T3 cells / D3 cells in the (A)/(C)/(E)/(G) absence and (B)/(D)/(F)/(H) presence of immunizing peptide using GTX48548 DNMT1 antibody [60B1220.1]. Dilution : 5 μg/ml for cells / 0.1μg/ml for recombinant protein
	FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Blue : Primary antibody Orange : isotype controlFixation : 4% PFA
	IHC-P analysis of human hepatocellular carcinoma tissue using GTX48548 DNMT1 antibody [60B1220.1]. Dilution : 1:50

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	60B1220.1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, MS, IHC (Free Floating)
Reactivity	Human, Mouse, Rat, Pig

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.1 - 0.5 μg/ml
ICC/IF	1:10
IHC-P	1:50 - 1:500
FACS	Assay dependent
IP	1:10 - 1:500
ChIP assay	1:20 - 1:1000
MS	Assay dependent
IHC (Free Floating)	1:500

Not tested in other applications.

Calculated MW	183 kDa. ( Note )

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS, 0.05% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI
Cellular Localization	Nucleus
Background	DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database	・ Gene ID: 1786 DNMT1 ・ UniProt: P26358 DNMT1

目次に戻る