抗DNMT3A抗体 | Anti-DNMT3A antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human testis tissue using GTX30365 DNMT3A antibody.
	ICC/IF analysis of NTERA-2 cells using GTX30365 DNMT3A antibody. Green : primary antibodyRed : beta-TubulinBlue : DAPI
	WB analysis of HCT116 mock (Lane 1) or overexpressing DNMT3A (Lane 2) nuclear extracts using GTX30365 DNMT3A antibody. Loading : 40μg

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000
ICC/IF	1:500
IHC-P	1:200
IP	1:10 - 1:500

Not tested in other applications.

Calculated MW	102 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	Serum, 0.1% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Amino acids 10-118 of human Dnmt3a [UniProt# Q9Y6K1]
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS
Cellular Localization	Nucleus,Cytoplasm
Background	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database	・ Gene ID: 1788 DNMT3A ・ UniProt: Q9Y6K1 DNMT3A

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Ratovitski EA et al. Cell Cycle 2014; 13 (5) : 749-61 Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.
Robert MF et al. Nat Genet 2003; 33 (1):22402 DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
Beaulieu N et al. J Biol Chem 2002; 277 (31):28176-81 An essential role for DNA methyltransferase DNMT3B in cancer cell survival.

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