抗DNMT1抗体 | Anti-DNMT1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of HeLa cells using GTX30364 DNMT1 antibody. Green : primary antibodyRed : TubulinBlue : DAPI Dilution : 1:50
	WB analysis of HEK293 cell lysate using GTX30364 DNMT1 antibody.
	IHC-P analysis of mouse spleen tissue using GTX30364 DNMT1 antibody.
	WB analysis of T24 cell lysate treated with 0.5 uM 5-Aza-Deoxycytidine using GTX30364 DNMT1 antibody. Loading : 50μgLane 1 : untreated T24 degrees cellsLane 2 : cell lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days
	IHC-P analysis of mouse small intestine tissue using GTX30364 DNMT1 antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000
ICC/IF	1:50 - 1:200
IHC-P	1:100
IP	1:10 - 1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW	183 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	Serum
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Contained within amino acids 1-125 of the N-terminus of human Dnmt1 [UniProt# P26358]
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI
Cellular Localization	Nucleus
Background	DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database	・ Gene ID: 1786 DNMT1 ・ UniProt: P26358 DNMT1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Li Y et al. Transl Oncol 2020; 13 (1) : 113-121 Silencing of CHFR Sensitizes Gastric Carcinoma to PARP Inhibitor Treatment.	Application : ChIP assay Reactivity : Human
Ratovitski EA et al. Cell Cycle 2014; 13 (5) : 749-61 Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.
Robert MF et al. Nat Genet 2003; 33 (1):22402 DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
Beaulieu N et al. J Biol Chem 2002; 277 (31):28176-81 An essential role for DNA methyltransferase DNMT3B in cancer cell survival.

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