抗HNF1B抗体(Anti-HNF1B Antibody )

• 価格
• 特長
• 使用例
• 製品情報

価格

目次に戻る

特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

目次に戻る

使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF1B (RC204453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit poly. Positive lysates LY400162 (100ug) and LC400162 (20ug) can be purchased separately from OriGene.
	Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HNF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA890005)(1:150)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HNF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA890005)(1:150)

目次に戻る

製品情報

Applications	WB, IHC
Recommend Dilution	WB 1:500, IHC 1:150,
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 50-150 of Human HNF1B.
Isotype	IgG
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Affinity purified
Predicted Protein Size	61.1 kDa
Gene Name	HNF1 homeobox B
Background	This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009].
Synonyms	FJHN|HNF-1-beta|HNF-1B|HNF1beta|HNF2|HPC11|LF-B3|LFB3|MODY5|TCF-2|TCF2|VHNF1
Database Link	NP_000449 Entrez Gene 6928 Human

目次に戻る