抗CSTF1 GAT Polyclonal抗体(Anti-CSTF1 GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

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使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSTF1 (RC219441, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSTF1. Positive lysates LY425563 (100ug) and LC425563 (20ug) can be purchased separately from OriGene.
	Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
	ELISA: cleavage stimulation factor Antibody
	Immunohistochemical staining of human gall bladder shows strong nuclear staining with an additional slightly weaker cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
	Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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製品情報

Clone Name	Polyclonal
Applications	WB, ELISA, IHC, IF
Recommend Dilution	WB 1:5000~20000,ELISA 1:100-1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	1.04mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	cleavage stimulation factor subunit 1
Background	This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]
Synonyms	CstF-50|CstFp50
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_001028693 Entrez Gene 1477 Human

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