抗TIE1 GAT Polyclonal抗体(Anti-TIE1 GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

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使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIE1 (RC207771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIE1. Positive lysates LY417318 (100ug) and LC417318 (20ug) can be purchased separately from OriGene.
	ELISA: TIE1 Antibody

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製品情報

Clone Name	Polyclonal
Applications	WB, ELISA
Recommend Dilution	WB 1:5000~20000, ELISA 1:100-1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the Middle Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	0.98mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
Background	TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
Synonyms	JTK14|TIE
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_005415 Entrez Gene 7075 Human

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