抗LHX1 GAT Polyclonal抗体(Anti-LHX1 GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

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使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX1 (RC210977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX1. Positive lysates LY417207 (100ug) and LC417207 (20ug) can be purchased separately from OriGene.
	Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LHX1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
	Western Blot: LIM1 Antibody - Western Blot was performed using affinity purified SEQer LHX1 antibody, aa(267-366) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer LHX1 antibody, aa(267-366) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
	ELISA: LIM1 Antibody
	Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
	Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
	Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
	Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)

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製品情報

Clone Name	Polyclonal
Applications	WB, ELISA, IHC
Recommend Dilution	WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Reactivity	Human , Monkey , Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	1mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	LIM homeobox 1
Background	This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.
Synonyms	LIM-1|LIM1
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_005559 Entrez Gene 3975 HumanEntrez Gene 16869 MouseEntrez Gene 714101 Monkey

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