抗PAX5 GAT Polyclonal抗体(Anti-PAX5 GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

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使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX5 (RC222785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX5. Positive lysates LY413830 (100ug) and LC413830 (20ug) can be purchased separately from OriGene.
	ELISA: PAX5 Antibody
	Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PAX5 Rabbit Polyclonal antibody. (TA590505)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PAX5 Rabbit Polyclonal antibody. (TA590505)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PAX5 Rabbit Polyclonal antibody. (TA590505)
	Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PAX5 Rabbit Polyclonal antibody. (TA590505)

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製品情報

Clone Name	Polyclonal
Applications	WB, ELISA, IHC
Recommend Dilution	WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the Middle Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	1.26mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	paired box 5
Background	This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
Synonyms	ALL3|BSAP
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_057953 Entrez Gene 5079 Human

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