抗ING1 GAT Polyclonal抗体(Anti-ING1 GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

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使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ING1 (RC210888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ING1. Positive lysates LY403677 (100ug) and LC403677 (20ug) can be purchased separately from OriGene.
	Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ING1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
	Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
	ELISA: ING1 Antibody
	Immunohistochemical staining of human gall bladder shows nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
	Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & plasma membrane.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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製品情報

Clone Name	Polyclonal
Applications	WB, ELISA, IHC, IF
Recommend Dilution	WB 1:5000~20000,ELISA 1:100-1:2000
Reactivity	Human , Rat , Monkey , Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the Middle Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	0.98mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	inhibitor of growth family member 1
Background	p33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodes the longest isoform (D). Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.
Synonyms	p24ING1c|p33|p33ING1|p33ING1b|p47|p47ING1a
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_937861 Entrez Gene 3621 HumanEntrez Gene 26356 MouseEntrez Gene 306626 RatEntrez Gene 694955 Monkey

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