抗MCM6 GAT Polyclonal抗体(Anti-MCM6 GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

目次に戻る

特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

目次に戻る

使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM6 (RC207097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM6. Positive lysates LY401786 (100ug) and LC401786 (20ug) can be purchased separately from OriGene.
	Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MCM6 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
	ELISA: MCM6 Antibody
	Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)
	Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)

目次に戻る

製品情報

Clone Name	Polyclonal
Applications	WB, ELISA, IHC
Recommend Dilution	WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
Reactivity	Human , Dog , Monkey , Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	1.07664 mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	minichromosome maintenance complex component 6
Background	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
Synonyms	MCG40308|Mis5|P105MCM
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_005906 Entrez Gene 4175 HumanEntrez Gene 17219 MouseEntrez Gene 707651 MonkeyEntrez Gene 476131 Dog

目次に戻る