抗FH GAT Polyclonal抗体(Anti-FH GAT Antibody Polyclonal)

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

TrueMAB抗体について
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。

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使用例

	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (RC200614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH. Positive lysates LY400053 (100ug) and LC400053 (20ug) can be purchased separately from OriGene.
	Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
	ELISA: Fumarase Antibody
	Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
	Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
	Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
	Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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製品情報

Clone Name	Polyclonal
Applications	WB, ELISA, IHC, IF
Recommend Dilution	WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the Middle Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	0.79mg/ml
Purification	The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Gene Name	fumarate hydratase
Background	The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
Synonyms	FMRD|HLRCC|LRCC|MCL|MCUL1
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Database Link	NP_000134 Entrez Gene 2271 Human

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