HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2. Positive lysates LY402871 (100ug) and LC402871 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-FCGR2A monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500645)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500645)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500645)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500645)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500645)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500645)
Anti-FCGR2A mouse monoclonal antibody (TA500645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A(RC205786).
Immunoprecipitation(IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size
34.7 kDa
Gene Name
Fc fragment of IgG receptor IIa
Background
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq].