Gas6 組換え体(リコンビナント)タンパク質 | Recombinant Human Gas6

• バルク注文に関して
• CF(Carrier-Free)とは？
• Product Details
• Additional Information
• Citations

バルク注文に関して

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CF(Carrier-Free)とは？

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Product Details

Source

Mouse myeloma cell line, NS0-derived, Ala49-Ala678, with a C-terminal 6-His tag

Accession

NP_000811

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

70.5 kDa

SDS-PAGE

70-85 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using T98G human glioblastoma cells.The ED50 for this effect is typically 100-400 ng/mL. Measured by its binding ability in a functional ELISA.When Recombinant Human Axl Fc Chimera (Catalog # 154-AL) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Gas6 that produces 50% of the optimal binding response is approximately 2.5‑15 ng/mL.

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Additional Information

Molecule Information

Gas6

Aliases

AXLLG

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Background

Gas6

Gas6 (Growth Arrest Specific 6) is one of six structurally unrelated proteins that are secreted by awide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein Sare vitamin K-dependent and have an extensively gamma-carboxylated N-terminal Gla domain, four EGF like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG).Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6. Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors. Gas6 induces a variety of responses, including prevention of apoptosis, cell proliferation, platelet-mediated thrombosis, retinal epithelial cell phagocytosis of outer rod segments, inhibition of VEGF-induced endothelial cell chemotaxis, and the differentiation and expansion of NK cell precursors. The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells.

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Citations

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