TIM-1/KIM-1/HAVCR 組換え体(リコンビナント)タンパク質 | Recombinant Rat TIM-1/KIM-1/HAVCR

• バルク注文に関して
• CF(Carrier-Free)とは？
• Product Details
• Additional Information
• Citations

バルク注文に関して

目次に戻る

CF(Carrier-Free)とは？

目次に戻る

Product Details

Source

Mouse myeloma cell line, NS0-derived, Ser18-Val238, with a C-terminal 6-His tag

Accession

O54947

Structure

Monomer

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

25.4 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and rrKIM-1.The ED50 for this effect is typically 0.4‑2 µg/mL.Optimal dilutions should be determined by each laboratory for each application.

目次に戻る

Additional Information

Molecule Information

TIM-1/KIM-1/HAVCR

Aliases

HAVCR1; KIM-1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat)

Background

TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2 cell mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain. The ECD contains one V-type Ig like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation.The TIM-1 gene is highly polymorphic and undergoes alternate splicing. For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A. There are two cytoplasmic alternate splice forms of TIM-1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in TIM-1a. TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule. The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin. TIM1 ligation induces T cell proliferation and promotes cytokine production.

目次に戻る

Citations

目次に戻る