Thrombospondin-1 組換え体(リコンビナント)タンパク質 | Recombinant Human Thrombospondin-1

• バルク注文に関して
• CF(Carrier-Free)とは？
• Product Details
• Additional Information
• Citations

バルク注文に関して

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CF(Carrier-Free)とは？

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Product Details

Source

Mouse myeloma cell line, NS0-derived, Asn19-Pro1170, with a C-terminal 10-His tag

Accession

P07996

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

129 kDa

SDS-PAGE

165-170 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.When 2 x 104 cells/well are added to DTT treated THBS-1 coated plates (10 µg/mL, 100 µL/well), > 50% will adhere after 1 hour at 37 °C.

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Additional Information

Molecule Information

Thrombospondin-1

Aliases

THBS1; TSP-1

Entrez Gene IDs

7057 (Human); 21825 (Mouse)

Background

Thrombospondin-1, -2, and -4

Thrombospondins (TSP) are secreted multidomain glycoproteins with many putative functions including modulating cell adhesion, proliferation, migration, and angiogenesis. At least five TSPs exist: TSP-1, -2, -3, -4, and cartilage oligomeric glycoprotein (COMP)/TSP-5. The family can be divided into two subgroups. The first, TSP-1 and -2, are homotrimeric molecules, each monomer containing an N-terminal/heparin-binding domain, a coiled-coil oligomerization domain, a von Willebrand factor-type/procollagen homology domain, three type 1 thrombospondin repeats (TSR), three EGF-like repeats, seven TSP type 3 repeats, and a C-terminal globular domain. TSP-1 and -2 are thought to mediate interactions between cells and the extracellular matrix, and as such are matricellular proteins. In contrast, TSP-3, -4, and COMP/TSP-5 are homopentamers that lack the N-terminal structure of TSP-1 and -2, but have a similar arrangement of EGF and TSP type 3 repeats, and the C-terminal domain characteristic of the TSP family.

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Citations

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