TIM-1/KIM-1/HAVCR 組換え体(リコンビナント)タンパク質 | Recombinant Mouse TIM-1/KIM-1/HAVCR

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• CF(Carrier-Free)とは？
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• Citations

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Product Details

Source

Mouse myeloma cell line, NS0-derived, Tyr22-Thr212, with a C-terminal 6-His tag

Accession

Q3V033

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

21.8 kDa

SDS-PAGE

40 - 50 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 or TNF-alphasecretion by RAW 264.7 mouse monocyte/macrophage cells.The ED50 for this effect is typically 0.1 - 0.4 µg/mL.Measured by its ability to bind THP‑1 human acute monocytic leukemia cells.As determined by flow cytometric analysis, there is a greater than 3 fold increase in the fluorescence of apoptotic THP-1 cells treated with 0.5 µg/mL recombinant mouse TIM-1/His and Mouse Anti‑polyHistidine PE-conjugated Monoclonal Antibody (R&D Systems, Catalog # IC050P) compared to cells stained with anti‑Mouse Anti‑polyHistidine PE-conjugated Monoclonal Antibody alone.

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Additional Information

Molecule Information

TIM-1/KIM-1/HAVCR

Aliases

HAVCR1; KIM-1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat)

Background

TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2 cell mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain. The ECD contains one V-type Ig like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation.The TIM-1 gene is highly polymorphic and undergoes alternate splicing. For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A. There are two cytoplasmic alternate splice forms of TIM-1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in TIM-1a. TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule. The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin. TIM1 ligation induces T cell proliferation and promotes cytokine production.

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Citations

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