B7-H1 Fc Chimera 組換え体(リコンビナント)タンパク質 | Recombinant Mouse B7-H1 Fc Chimera
掲載日情報:2015/01/26 現在Webページ番号:111419
R&D Systems社製の高品質なB7-H1 Fc Chimeraの組換え体タンパク質(Recombinant Mouse B7-H1 Fc Chimera)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年04月26日 12時55分現在]
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B7-H1/Fc Chimera, Mouse, Recombinant |
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[在庫・価格 :2025年04月26日 12時55分現在]
B7-H1/Fc Chimera, Mouse, Recombinant
文献数: 8
- 商品コード:1019-B7-100
- メーカー:RSD
- 包装:100μg
- 価格:¥85,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 産生:NS0,純度:>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.,由来動物:Mouse,M.W.:51.3 kDa (monomer),Genbank:29126,エンドトキシンレベル:<0.10 EU per 1 µg of the protein by the LAL method. 別名:B7-H Genbank No: 29126 Protein Accession No: Q9EP73 |
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保存条件 | -20℃ | 法規備考 | |
掲載カタログ |
ニュース2018年7月1日号 p.6
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製品記事 | R&D SystemsのB7-H1/PD-L1関連製品 |
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関連記事 |
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バルク注文に関して
※バルク包装品も承ります。お気軽にお問合せ下さい。
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CF(Carrier-Free)とは?
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Source
- Mouse myeloma cell line, NS0-derived
- Accession
- Q9EP73
- Structure
- Disulfide-linked homodimer
- Purity
- >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Predicted Molecular Mass
- 51.3 kDa (monomer)
- SDS-PAGE
- 75-85 kDa, reducing conditions
- Activity
- Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells.The ED50 for this effect is typically 0.15-0.75 µg/mL.
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Additional Information
- Molecule Information
- B7-H1/PD-L1
- Aliases
- CD274; PDCD1L1; PDCD1LG1; PD-L1
- Entrez Gene IDs
- 29126 (Human); 60533 (Mouse)
- Background
- B7-H1
- Human B7 homolog 1 (B7-H1), also called programmed cell death 1 ligand 1 (PDCD1L1) and programmed death ligand 1 (PD-L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors.The B7-H1 gene encodes a 290 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
Butte MJ, Freeman GJ, Keir ME, Phamduy TB, Sharpe AH
Immunity 2007 27:111-22
Species: N/A
Protein Application: Surface Plasmon Resonance
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