Mouse Vitamin D BP Quantikine ELISA Kit | mVitD BP QKit
掲載日情報：2016/08/18 現在Webページ番号：111366
R&D Systems製のMouse Vitamin D BPを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Mouse Vitamin D BP Quantikine ELISA Kit |
|
本製品は取扱中止になりました | 0 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月27日 00時00分現在]
Mouse Vitamin D BP Quantikine ELISA Kit
文献数： 0
- 商品コード：MVDBP0
- メーカー：RSD
- 包装：1kit
- 本製品は取扱中止になりました
| 説明文 | |||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
|
||
| 製品記事 | Quantikine Mouse Vitamin D Binding Protein ELISA Kit |
||
| 関連記事 | |||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Tissue Lysates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
- Sensitivity
- 0.033 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine, Tissue Lysates)
- Specificity
- Natural and recombinant mouse Vitamin D BP.
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Mouse Vitamin D BP Immunoassay is a 2.5 hour solid phase ELISA designed to measure mouse Vitamin D BP levels in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Vitamin D BP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse Vitamin D BP accurately. Results obtained using natural mouse Vitamin D BP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Vitamin D BP.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.404 | 1.13 | 3.3 | 0.444 | 1.22 | 3.47 |
| Standard Deviation | 0.02 | 0.04 | 0.082 | 0.027 | 0.056 | 0.148 |
| CV% | 5 | 3.5 | 2.5 | 6.1 | 4.6 | 4.3 |
追加しました。
Recovery
The recovery of mouse Vitamin D BP spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 98 | 94-105 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Vitamin D BP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
追加しました。
Additional Infomation
- Molecule Information
- Vitamin D BP
- Aliases
- DBP; DBP-MAF; Gc; Gc-MAF; VDBG; VDBP
- Entrez Gene IDs
- 2638 (Human); 14473 (Mouse); 24384 (Rat)
- Background
- Vitamin D Binding Protein
- Vitamin D BP, also called VDB, DBP, Group Specific Component (Gc) or macrophage-activating factor (DBP- or Gc-MAF), is a member of the albumin family. These proteins circulate in the blood, are produced by the liver, have a U-shaped molecular structure due to a conserved pattern of cysteine motifs, and show ligand/carrier transport function. VitD BP functions to transport Vitamin D, activate macrophages and osteoclasts, and enhance C5a chemotactic activity.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。