"Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit," | mIL-1b Qkit
掲載日情報：2016/08/18 現在Webページ番号：111315
R&D Systems製の"Mouse IL-1 beta/IL-1F2"を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月30日 08時15分現在]
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IL-1β, Mouse, ELISA Kit, Quantikine, 1Plate <IL-1F2, ELISA Kit> |
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[在庫・価格 ：2024年04月30日 08時15分現在]
IL-1β, Mouse, ELISA Kit, Quantikine, 1Plate <IL-1F2, ELISA Kit>
文献数： 0
- 商品コード：MLB00C-1
- メーカー：RSD
- 包装：1kit
- 価格：¥104,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：12.50 - 800 pg/mL，感度：4.8 pg/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-1 beta/IL-1F2 別名：catabolin Genbank No： 3553 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
R&D Systems社 IL-1β関連製品 |
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| 関連記事 |
R&D Systems マウス／ラット用Quantikine ELISA Kit 2 plate製品の包装変更について R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 4.8 pg/mL
- Assay Range
- 12.5 - 800 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse IL-1 beta
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 33.5 | 92.9 | 386 | 34.6 | 93.5 | 423 |
| Standard Deviation | 2.5 | 4.3 | 11.7 | 2.9 | 6.2 | 23.9 |
| CV% | 7.5 | 4.6 | 3 | 8.4 | 6.6 | 5.7 |
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Recovery
The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 106 | 95-119 |
| Cell Lysates (n=4) | 115 | 110-120 |
| Serum (n=4) | 105 | 98-113 |
| EDTA Plasma (n=4) | 108 | 101-117 |
| Heparin Plasma (n=4) | 97 | 87-114 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-1 beta/IL-1F2
- Aliases
- IL1B; IL-1F2
- Entrez Gene IDs
- 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 100034237 (Equine); 100135556 (Guinea Pig)
- Background
- IL-1 beta/IL-1F2
- IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice.
Axt D, Delekate A, Gelpi E, Golenbock D, Griep A, Halle A, Heneka M, Korte M, Kummer M, Latz E, Remus A, Schwartz S, Stutz A, Tzeng T, Vieira-Saecker A
Nature 2013 493:674-8
Species: Mouse
Sample Type: Tissue Homogenates
Sirt2 suppresses inflammatory responses in collagen-induced arthritis.
Hong H, Jiang C, Lin J, Sun B, Zheng Y
Biochem Biophys Res Commun 2013 441:897-903
Species: Mouse
Sample Type: Serum
Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
Aritake K, Hirai H, Nagata K, Nakamura M, Narumiya S, Otani S, Satoh T, Urade Y, Yamamoto Y, Yokozeki H
Am. J. Pathol. 2012 179:302-14
Species: Mouse
Sample Type: Tissue Homogenates
Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
Chen Q, Deng T, Han D, Yan K, Zhang Y
Immunology 2012 135:40-50
Species: Mouse
Sample Type: Cell Culture Supernates
Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
Gottfries J, Melgar S, Michaelsson E
PLoS ONE 2012 7:e30005
Species: Mouse
Sample Type: Tissue Homogenates
The axonal guidance receptor neogenin promotes acute inflammation.
Gatidou D, Granja T, Konig K, Meier J, Mirakaj V, Rosenberger P
PLoS ONE 2012 7:e32145
Species: Mouse
Sample Type: Peritoneal Lavage
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Dervish S, Fulcher G, Harrison LC, Jackson CJ, Xue M
J. Biol. Chem. 2012 287:16356-64
Species: Mouse
Sample Type: Cell Culture Supernates
Netrin-1 protects against L-Arginine-induced acute pancreatitis in mice.
Cai Q, Chen J, Chen X, Fu H, Shen P, Wang C, Yan R, Yang D
PLoS ONE 2012 7:e46201
Species: Mouse
Sample Type: Plasma
Exendin-4 protected against cognitive dysfunction in hyperglycemic mice receiving an intrahippocampal lipopolysaccharide injection.
Chen Y, Hsieh-Li H, Huang H, Jhao J, Jheng Y, Lee-Chen G, Liang K, Su M
PLoS ONE 2012 7:e39656
Species: Mouse
Sample Type: Plasma
Treatment of diabetic mice with undenatured whey protein accelerates the wound healing process by enhancing the expression of MIP-1alpha, MIP-2, KC, CX3CL1 and TGF-beta in wounded tissue.
BMC Immunol. 2012 13:32
Species: Mouse
Sample Type: Tissue Homogenates
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