"Mouse/Rat CCL2/JE/MCP-1 Quantikine ELISA Kit, 2 Plate" | m/rCCL2/JE Qkit
掲載日情報：2016/08/18 現在Webページ番号：111310
R&D Systems製の"Mouse/Rat CCL2/JE/MCP-1, 2 Plate"を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月17日 00時00分現在]
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MCP-1, Mouse/Rat, ELISA Kit, Quantikine M (2×96well) <JE, ELISA Kit> |
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[在庫・価格 ：2024年04月17日 00時00分現在]
MCP-1, Mouse/Rat, ELISA Kit, Quantikine M (2×96well) <JE, ELISA Kit>
文献数： 217
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 法規備考 | ||
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| 関連記事 |
R&D Systems社#MJE00／SMJE00／PMJE00の販売終了および改良版#MJE00B／SMJE00B／PMJE00Bの販売のお知らせ |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Serum (25 µL)
- Sensitivity
- 2 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Cell Culture Supernates)
- Specificity
- Natural and recombinant mouse and rat MCP-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat CCL2/MCP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-1 in mouse or rat cell culture supernates and serum. It contains E. coli-expressed mouse MCP-1 and antibodies raised against the recombinant factor. This Immunoassay has been shown to accurately quantitate recombinant mouse MCP-1. Results obtained using natural mouse or rat MCP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural MCP-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 47.1 | 311 | 768 | 43.6 | 310 | 783 |
| Standard Deviation | 3.9 | 15.8 | 40.6 | 3.2 | 14.3 | 42.8 |
| CV% | 8.3 | 5.1 | 5.3 | 7.3 | 4.6 | 5.5 |
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Recovery
The recovery of MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Mouse Cell Culture Supernates (n=6) | 104 | 91-119 |
| Rat Cell Culture Supernates (n=4) | 109 | 103-115 |
| Mouse Serum (n=9) | 99 | 89-113 |
| Rat Serum (n=4) | 109 | 103-116 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MCP-1 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- CCL2/JE/MCP-1
- Aliases
- MCAF; MCP-1
- Entrez Gene IDs
- 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
- Background
- CCL2/MCP-1/JE
- MCP-1, also known as monocyte chemotactic and activating factor (MCAF), was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Sirt2 suppresses inflammatory responses in collagen-induced arthritis.
Hong H, Jiang C, Lin J, Sun B, Zheng Y
Biochem Biophys Res Commun 2013 441:897-903
Species: Mouse
Sample Type: Serum
Monocyte-derived dendritic cell recruitment and allergic T(H)2 responses after exposure to diesel particles are CCR2 dependent.
Joos GF, Maes T, Provoost S, Tournoy KG
J. Allergy Clin. Immunol. 2012 129:483-91
Species: Mouse
Sample Type: BALF
Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
Gottfries J, Melgar S, Michaelsson E
PLoS ONE 2012 7:e30005
Species: Mouse
Sample Type: Tissue Homogenates
The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
Risingsong R, Royce D, Tran K
Cancer Prev Res (Phila) 2012 5:726-34
Species: Mouse
Sample Type: Cell Culture Supernates
Tissue factor promotes activation of coagulation and inflammation in a mouse model of sickle cell disease.
Chantrathammachart P, Mackman N, Sparkenbaugh E
Blood 2012 120:636-46
Species: Mouse
Sample Type: Tissue Homogenates
Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor.
J. Exp. Med. 2012 209:1493-503
Species: Mouse
Sample Type: Cell Culture Supernates
Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
J. Immunol. 2012 189:2545-52
Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice.
Mol. Cell. Endocrinol. 2012 358:127-34
Inhalative vs. systemic IL-10 administration: differences in the systemic inflammatory response and end-organ inflammation following hemorrhagic shock.
Dombroski D, Kobbe P, Lichte P, Pape H, Pfeifer R, Schmidt J, Schreiber H, Sellei R
Cytokine 2012 60:266-70
Abscisic acid regulates inflammation via ligand-binding domain-independent activation of peroxisome proliferator-activated receptor gamma.
Bassaganya-Riera J, Bevan DR, Carbo A, Climent M, Guri AJ, Hontecillas R, Horne WT, Lewis SN, Lu P, Sobral BW
J. Biol. Chem. 2011 286:2504-16
Species: Mouse
Sample Type: Cell Culture Supernates
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