Mouse Gas6 Quantikine ELISA Kit | mGas6 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111302
R&D Systems製のMouse Gas6を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2026年08月05日 20時55分現在]
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[在庫・価格 ：2026年08月05日 20時55分現在]
Mouse Gas6 Quantikine ELISA Kit
文献数： 0
- 商品コード：MGAS60
- メーカー：RSD
- 包装：1kit
- 本製品は取扱中止になりました
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.027 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse Gas6
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse Gas6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Gas6 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse Gas6 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Gas6 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Gas6.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.451 | 1.28 | 3.93 | 0.465 | 1.3 | 3.98 |
| Standard Deviation | 0.023 | 0.052 | 0.129 | 0.024 | 0.054 | 0.285 |
| CV% | 5.1 | 4.1 | 3.3 | 5.2 | 4.2 | 7.2 |
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Recovery
The recovery of Gas6 spiked into cell culture samples were evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 111 | 103-118 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Gas6 were serially diluted with Calibrator Diluent RD5-26 (1X) to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Gas6
- Aliases
- AXLLG
- Entrez Gene IDs
- 2621 (Human); 14456 (Mouse)
- Background
- Gas6
- Gas6 (Growth Arrest Specific 6) is one of six structurally unrelated proteins that are secreted by awide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein Sare vitamin K-dependent and have an extensively gamma-carboxylated N-terminal Gla domain, four EGF like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG).Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6. Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors. Gas6 induces a variety of responses, including prevention of apoptosis, cell proliferation, platelet-mediated thrombosis, retinal epithelial cell phagocytosis of outer rod segments, inhibition of VEGF-induced endothelial cell chemotaxis, and the differentiation and expansion of NK cell precursors. The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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