Human IL-6 Quantikine HS ELISA Kit | hIL-6 QKit HS Kit
掲載日情報：2016/08/18 現在Webページ番号：111237
R&D Systems製のHuman IL-6 Quantikine HS ELISA Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月20日 00時00分現在]
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IL-6, Human, ELISA Kit, Quantikine HS, 2nd Generation (96well) |
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[在庫・価格 ：2024年04月20日 00時00分現在]
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5.5 hours
- Sample Type & Volume Required Per Well
- Serum (100 µL), EDTA Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
- Sensitivity
- 0.11 pg/mL
- Assay Range
- 0.156 - 10 pg/mL (Serum, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human IL-6
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine HS Human IL-6 Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human IL-6 in serum, plasma, and urine. It contains E. coli-expressed recombinant human IL-6 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IL-6.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 36 | 36 | 36 |
| Mean | 0.436 | 2.45 | 5.53 | 0.49 | 2.78 | 5.65 |
| Standard Deviation | 0.03 | 0.19 | 0.41 | 0.047 | 0.2 | 0.37 |
| CV% | 6.9 | 7.8 | 7.4 | 9.6 | 7.2 | 6.5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 35 | 35 | 35 |
| Mean | 0.526 | 3.09 | 5.77 | 0.502 | 2.78 | 5.62 |
| Standard Deviation | 0.052 | 0.18 | 0.32 | 0.056 | 0.19 | 0.31 |
| CV% | 9.8 | 5.8 | 5.5 | 11.2 | 6.8 | 5.5 |
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Recovery
The recovery of IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=8) | 94 | 87-99 |
| EDTA Plasma (n=8) | 97 | 84-113 |
| Citrate Plasma (n=8) | 106 | 90-125 |
| Urine (n=8) | 102 | 93-112 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-6
- Aliases
- BSF-2; IFN-beta 2; MGI-2A
- Entrez Gene IDs
- 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
- Background
- IL-6
- Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
Aoudjit F, Boisvert M, Chakir J, Fakhfakh R, Hadj-Salem I, Jacques E, Loubaki L, Plante S
PLoS ONE 2013 8:e81983
Species: Human
Sample Type: Cell Culture Supernates
Exenatide as a weight-loss therapy in extreme pediatric obesity: a randomized, controlled pilot study.
Abuzzahab MJ, Billington CJ, Deering MM, Fitch AK, Fox CK, Gandrud LM, Kelly AS, Metzig AM, Moran A, Nathan BM, Rudser KD, Schwartz BL, Schwarzenberg SJ
Obesity (Silver Spring) 2012 20:364-70
Species: Human
Sample Type: Plasma
Elderly trauma patients have high circulating noradrenaline levels but attenuated release of adrenaline, platelets, and leukocytes in response to increasing injury severity.
Johansson PI, Larsen CF, Ostrowski SR, Perner A, Sorensen AM, Wanscher M, Welling KL
Crit. Care Med. 2012 40:1844-50
Species: Human
Sample Type: Plasma
Regulation of hepcidin expression at high altitude.
Dorrington KL, Lakhal S, Leon-Velarde F, Mole DR, Nickol AH, Privat C, Rivera-Ch M, Robbins PA, Smith TG, Talbot NP
Blood 2012 119:857-60
Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
Buch MH, Church LD, Churchman SM, Emery P, Geiler J, Horner EA, McDermott MF, Parmar R, Ponchel F
Clin. Exp. Rheumatol. 2012 30:534-42
Species: Human
Sample Type: Serum
Administration of omega-3 fatty acids and Raloxifene to women at high risk of breast cancer: interim feasibility and biomarkers analysis from a clinical trial.
Eur J Clin Nutr 2012 66:878-84
Species: Human
Sample Type: Serum
Exercise induces a marked increase in plasma follistatin: evidence that follistatin is a contraction-induced hepatokine.
Brandt C, Febbraio MA, Hansen J, Hojman P, Nielsen AR, Pedersen BK, Plomgaard P, Whitham M
Endocrinology 2011 152:164-71
Species: Human
Sample Type: Plasma
Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
Bertini E, Bianchi ML, Capulli M, Carvello F, De Benedetti F, De Pasquale L, De Simone M, Del Fattore A, Ferrari S, Morandi L, Pierroz D, Rucci N, Rufo A, Teti A
J. Bone Miner. Res. 2011 26:1891-903
Species: Human
Sample Type: Serum
Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise?
Grosset JF, Onambele-Pearson GL, Pledge D
Cytokine 2011 55:318-23
Species: Human
Sample Type: Serum
Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
Barbiche E, Bieuzen F, Fillard JR, Hausswirth C, Louis J, Pournot H
PLoS ONE 2011 6:e22748
Species: Human
Sample Type: Plasma
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