Human sVEGF R1/Flt-1 Quantikine ELISA Kit | hsVEGF R1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111230
R&D Systems製のHuman sVEGF R1/Flt-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Flt-1, Soluble, Human, ELISA Kit, Quantikine, 2nd Generation (96 well) <VEGF Receptor1, ELISA Kit> |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Flt-1, Soluble, Human, ELISA Kit, Quantikine, 2nd Generation (96 well) <VEGF Receptor1, ELISA Kit>
文献数： 51
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Saliva (100 µL), Follicular Fluid (100 µL)
- Sensitivity
- 13.3 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Saliva, Cell Culture Supernates, EDTA Plasma, Follicular Fluid)
- Specificity
- Natural and recombinant human soluble VEGF R1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human soluble VEGF R1 in cell culture supernates, serum, plasma, saliva, and follicular fluid. It contains Sf 21-expressed recombinant human VEGF R1/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring soluble VEGF R1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Follicular Fluid
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 96.6 | 596 | 1,213 | 112 | 645 | 1,279 |
| Standard Deviation | 2.5 | 22.8 | 39.3 | 11 | 44.9 | 70.5 |
| CV% | 2.6 | 3.8 | 3.2 | 9.8 | 7 | 5.5 |
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Recovery
The recovery of VEGF R1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 100 | 86-113 |
| Serum (n=4) | 103 | 96-107 |
| EDTA Plasma (n=4) | 99 | 89-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VEGF R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- VEGF R1/Flt-1
- Aliases
- FLT1; Flt-1
- Entrez Gene IDs
- 2321 (Human); 14254 (Mouse)
- Background
- VEGF R1, R3
- VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoieticdevelopment. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Cardiac angiogenic imbalance leads to peripartum cardiomyopathy.
Arany Z, Bauersachs J, Burke SD, del Monte F, Farrell C, Hacker MR, Hess P, Hilfiker-Kleiner D, Jang C, Karumanchi SA, Khankin EV, Koulisis N, Liu L, Mahmood F, Mitchell J, Patten IS, Rana S, Rhee JS, Rowe GC, Shahul S, Tudorache I
Nature 2012 485:333-8
Species: Human
Sample Type: Plasma
Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
Bork U, Buchler MW, Falk CS, Herber M, Hinz U, Koch M, Rahbari NN, Schmidt T, Weitz J
BMC Cancer 2011 11:286
Species: Human
Sample Type: Serum
Phase I and pharmacokinetic study of sunitinib in pediatric patients with refractory solid tumors: a children's oncology group study.
Adamson PC, Ahern CH, Baruchel S, Blaney SM, Dubois SG, Glade-Bender J, Grier HE, Ingle AM, Ivy P, Reid JM, Shusterman S, Wu B
Clin. Cancer Res. 2011 17:5113-22
Species: Human
Sample Type: Plasma
Prognostic significance of angiogenic factors in uterine cervical cancer.
Blohmer JU, Heidecke H, Jeschke S, Korlach S, Kummel S, Landt S, Lichtenegger W, Schmid P, Sehouli J, Stoblen F, Wehling M
Anticancer Res. 2011 31:2589-95
Species: Human
Sample Type: Serum
In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
Barinoff J, Heidecke H, Korlach S, Kummel S, Landt S, Reuter C, Schwidde I, Sehouli J, Thill M
Anticancer Res. 2011 31:2609-15
Species: Human
Sample Type: Serum
Soluble Flt-1 gene therapy ameliorates albuminuria but accelerates tubulointerstitial injury in diabetic mice.
Campbell-Thompson M, Chiodo VA, Croker BP, Grant M, Kosugi T, Li Q, Nakagawa T, Nakayama T, Zhang L
Am. J. Physiol. Renal Physiol. 2010 298:F609-16
Species: Human
Sample Type: Urine
Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
Adamyan L, Bourlev V, Iljasova N, Larsson A, Olovsson M
Fertil. Steril. 2010 94:52-7
Species: Human
Sample Type: Serum
An automated method for the determination of the sFlt-1/PIGF ratio in the assessment of preeclampsia.
Denk B, Dudenhausen JW, Galindo A, Herraiz I, Moertl MG, Pape J, Schlembach D, Stepan H, Verlohren S, Zeisler H
Am. J. Obstet. Gynecol. 2010 202:161.e1-161.e1
Species: Human
Sample Type: Serum
Soluble fms-Like tyrosine kinase 1 (sFlt1), endoglin and placental growth factor (PlGF) in preeclampsia among high risk pregnancies.
Clifton RG, Jeyabalan A, Powers RW
PLoS ONE 2010 5:e13263
Species: Human
Sample Type: Serum
Effect of smoking on circulating angiogenic factors in high risk pregnancies.
Bain RP, Brenner S, Caritis S, Carter B, Carter SG, Catz C, Christmas JT, Clifton RG, Collins B, Copper R, Cotroneo M, Elder N, Elder S, Fischer ML, Goldenberg R, Hauth JC, Iams JD, Jeyabalan A, Johnson D, Jones P, Kamon T, Klebanoff MA, Kuller J, Landon M, Lindheimer MD, MacPherson C, McCart D, McCoy S, McNellis D, Meadows S, Meier A, Meis P, Mercer B, Millinder A, Minton V, Miodovnik M, Moawad AH, Mueller E, Newman RB, Norman G, Paul R, Pemberton V, Powers RW, Rabello Y, Ramsey R, Roberts JM, Romero R, Sabo V, Sibai B, Sorokin Y, Spong C, Swain M, Thom E, Thurnau G, Van Dorsten P, Yaffe S
PLoS ONE 2010 5:e13270
Species: Human
Sample Type: Serum
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