Human Pentraxin 3/TSG-14 Quantikine ELISA Kit | hPTX3 Qkit
掲載日情報：2018/03/26 現在Webページ番号：111176
R&D Systems製のHuman Pentraxin 3/TSG-14を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年06月29日 00時00分現在]
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Pentraxin 3, Human, ELISA Kit, Quantikine <TSG-14, ELISA Kit> |
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[在庫・価格 ：2025年06月29日 00時00分現在]
Pentraxin 3, Human, ELISA Kit, Quantikine <TSG-14, ELISA Kit>
文献数： 15
追加しました。
- Product Details
- Product Summary
- Precision
- Recovery
- Linearity
- Natural Linearity
- Additional Infomation
- Citations
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5 hours
- Sample Type & Volume Required Per Well
- Serum-free Cell Culture Supernates (100 µL), EDTA Plasma (100 µL), Saliva (100 µL)
- Sensitivity
- 0.116 ng/mL
- Assay Range
- 0.31 - 20 ng/mL (Saliva, EDTA Plasma, Serum-free Cell Culture Supernates)
- Specificity
- Natural and recombinant human PTX3
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum-free Cell Culture Supernates, EDTA Plasma, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 2.61 | 7.72 | 14.1 | 2.75 | 7.94 | 14.5 |
| Standard Deviation | 0.01 | 0.28 | 0.62 | 0.17 | 0.39 | 0.6 |
| CV% | 3.8 | 3.7 | 4.4 | 6.1 | 5 | 4.1 |
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Recovery
The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| EDTA Plasma (n=4) | 99 | 83-106 |
| Serum-free Cell Culture Media (n=4) | 101 | 95-108 |
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Linearity
To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Natural Linearity
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Additional Infomation
- Molecule Information
- Pentraxin 3/TSG-14
- Aliases
- PTX3; TSG-14
- Entrez Gene IDs
- 5806 (Human); 19288 (Mouse)
- Background
- Pentraxin 3/TSG-14
- Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection.
Hurme M, Jylhava J, Kuparinen T, Leppanen S, Makela S, Mustonen J, Outinen TK, Porsti I, Syrjanen J, Vaheri A
PLoS ONE 2012 7:e31455
Species: Human
Sample Type: Plasma
Chemerin and adiponectin contribute reciprocally to metabolic syndrome.
Ahn KY, Chu SH, Im JA, Jeon JY, Lee DC, Lee JW, Lee MK, Park MS
PLoS ONE 2012 7:e34710
Species: Human
Sample Type: Plasma
Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
Chakir J, Gounni AS, Halayko AJ, Koussih L, Redhu NS, Shan L, Zhang J
PLoS ONE 2012 7:e34965
Species: Human
Sample Type: Cell Culture Supernates
Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
Carrero JJ, Davidge ST, Hammarqvist F, Kublickiene K, Luksha L, Stenvinkel P
PLoS ONE 2012 7:e36056
Species: Human
Sample Type: Serum
A multi-analyte assay for the non-invasive detection of bladder cancer.
Chang , Myron, Dai , Yunfeng, Goodison , Steve, Rosser , Charles , Urquidi , Virginia
PLoS ONE 2012 7:e47469
Species: Human
Sample Type: Urine
High Plasma Level of Long Pentraxin 3 (PTX3) Is Associated with Fatal Disease in Bacteremic Patients: A Prospective Cohort Study.
Aittoniemi J, Huhtala H, Hurme M, Huttunen R, Jylhava J, Laine J, Syrjanen J, Vuento R
PLoS ONE 2011 6:e17653
Species: Human
Sample Type: Plasma
Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
Diamandis EP, Goodglick L, Planque C, Thornquist MD
Clin. Cancer Res. 2011 17:2395-2399
Species: Human
Sample Type: Serum
Pentraxin 3 (PTX3) is associated with cardiovascular risk factors: the Health 2000 Survey.
Haarala A, Hurme M, Jula A, Jylhava J, Kahonen M, Kesaniemi YA, Lehtimaki T, Moilanen L, Nieminen MS
Clin. Exp. Immunol. 2011 164:211-7
Species: Human
Sample Type: Plasma
Is pentraxin 3 involved in obesity-induced decrease in arterial distensibility?
Maeda S, Miyaki A, Yoshizawa M
J. Atheroscler. Thromb. 2010 17:278-84
Species: Human
Sample Type: Plasma
Genetic variation in pentraxin (PTX) 3 gene associates with PTX3 production and fertility in women.
Kuningas M, May L, van Bodegom D
Biol. Reprod. 2010 82:299-304
Species: Human
Sample Type: Cell Culture Supernates
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