Human LIF Quantikine ELISA Kit | hLIF QKit
掲載日情報：2016/08/18 現在Webページ番号：111136
R&D Systems製のHuman LIFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月06日 00時00分現在]
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LIF, Human, ELISA Kit, Quantikine (96well) <Leukemia Inhibitory Factor, ELISA Kit> |
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[在庫・価格 ：2024年05月06日 00時00分現在]
LIF, Human, ELISA Kit, Quantikine (96well) <Leukemia Inhibitory Factor, ELISA Kit>
文献数： 17
| 説明文 |
Genbank No：
3976
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| 保存条件 | 法規備考 | ||
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| 関連記事 |
R&D Systems社製品#DLF00、QC113の販売終了および改良版#DLF00B、QC256の販売 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours or 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL)
- Sensitivity
- 8 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human LIF
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human LIF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure soluble human LIF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human LIF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant LIF accurately. Results obtained measuring natural human LIF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human LIF.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 314 | 868 | 1,347 | 87.4 | 524 | 1,017 |
| Standard Deviation | 7.7 | 24.3 | 23.8 | 6.2 | 34.9 | 46.5 |
| CV% | 2.5 | 2.8 | 1.8 | 7.1 | 6.7 | 4.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 341 | 875 | 1,358 | 81.6 | 472 | 910 |
| Standard Deviation | 12.3 | 34.5 | 42.5 | 4.4 | 17.7 | 35.2 |
| CV% | 3.6 | 3.9 | 3.1 | 5.4 | 3.8 | 3.9 |
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Recovery
The recovery of LIF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=11) | 102 | 91-110 |
| Heparin Plasma (n=8) | 92 | 84-100 |
| EDTA Plasma (n=2) | 89 | 84-97 |
| Cell Culture Media (n=4) | 97 | 91-106 |
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Additional Infomation
- Molecule Information
- LIF
- Aliases
- undefined
- Entrez Gene IDs
- 3976 (Human); 16878 (Mouse)
- Background
- Leukemia Inhibitory Factor (LIF) and Receptor
- Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
The role of p38 mitogen-activated protein kinase in serum-induced leukemia inhibitory factor secretion by bone marrow stromal cells from pediatric myelodysplastic syndromes.
Arantes C, Brentani MM, da Costa SV, Junqueira MS, Roela RA
Leuk. Res. 2010 34:507-12
Species: Human
Sample Type: Cell Culture Supernates
Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.
Cai B, Sun X, Xiao Y, Xue Q, Yang X, Zhang J, Zhou Y
Fertil. Steril. 2010 94:85-9
Species: Human
Sample Type: Flushed Uterine Fluid
Helicobacter pylori cytotoxin-associated gene A activates the signal transducer and activator of transcription 3 pathway in vitro and in vivo.
Ahn D, Ang M, Bronte-Tinkew DM, Franco A, Jones NL, Mimuro H, Peek RM, Ropeleski MJ, Sasakawa C, Terebiznik M
Cancer Res. 2009 69:632-9
Species: Human
Sample Type: Cell Culture Supernates
Prokineticin 1 mediates fetal-maternal dialogue regulating endometrial leukemia inhibitory factor.
Brown P, Catalano RD, Critchley HO, Evans J, Fazleabas AT, Jabbour HN, Sherwin R
FASEB J. 2009 23:2165-75
Species: Human
Sample Type: Cell Culture Supernates
Isoflavones regulate secretion of leukemia inhibitory factor and transforming growth factor {beta} and expression of glycodelin in human endometrial epithelial cells.
Ikeda K, Miki T, Pan WJ, Shiotani M, Watanabe J, Xu JW, Yamori Y, Yanaihara A, Yasui N
J. Endocrinol. 2008 196:425-33
Species: Human
Sample Type: Cell Culture Supernates
Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression.
Baba H, Ishiko T, Kamohara H, Ogawa M, Sakamoto K
Int. J. Oncol. 2007 30:977-83
Species: Human
Sample Type: Cell Culture Supernates
Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
Anegon I, Catala L, Delneste Y, Descamps P, Duluc D, Gamelin E, Gascan H, Grimaud L, Hebbar M, Ifrah N, Jeannin P, Lenoir J, Moles MP, Preisser L, Tan F
Blood 2007 110:4319-30
Species: Human
Sample Type: Cell Culture Supernates
Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
Brosnan CF, Einheber S, John GR, Melendez-Vasquez C, Raine CS, Salzer JL, Taveggia C, Zhang Y
J. Neurosci. 2006 26:12174-85
Species: Human
Sample Type: Cell Culture Supernates
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Bersinger NA, Birkhauser MH, Mueller MD, Wunder DM
Acta Obstet Gynecol Scand 2006 85:336-42
Species: Human
Sample Type: Follicular Fluid
Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
Fujio Y, Hamaguchi T, Hirota H, Izumi M, Kunisada K, Murakami E, Nakaoka Y, Oshima Y, Sugiyama S, Yamauchi-Takihara K
Heart Vessels 2004 19:237-41
Species: Human
Sample Type: Plasma
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