Human IFN-gamma Quantikine ELISA Kit | "hIFN-g QKit, 2nd Gen"
掲載日情報：2016/08/18 現在Webページ番号：111121
R&D Systems製のHuman IFN-gammaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月06日 00時00分現在]
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IFN-γ, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2025年05月06日 00時00分現在]
IFN-γ, Human, ELISA Kit, Quantikine (96 well)
文献数： 261
| 説明文 | Genbank No：
3458
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 法規備考 | ||
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| 製品記事 | |||
| 関連記事 | R&D Systems社製品#DIF50, SIF50, PDIF50の販売終了および改良版#DIF50C, SIF50C, PDIF50Cの販売 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL)
- Sensitivity
- 8 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human IFN-gamma
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 79.2 | 214 | 466 | 78.7 | 206 | 457 |
| Standard Deviation | 3.7 | 5.5 | 13 | 6.1 | 13.1 | 17 |
| CV% | 4.7 | 2.6 | 2.8 | 7.8 | 6.4 | 3.7 |
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Recovery
The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 93-109 |
| Serum (n=5) | 102 | 91-118 |
| EDTA Plasma (n=5) | 98 | 88-111 |
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Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IFN-gamma
- Aliases
- IFNG
- Entrez Gene IDs
- 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
- Background
- IFN-gamma
- Interferon-γ (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells.IFN-γ was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, up-regulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments Helper T cell expansion and may be required for Helper T cell differentiation.IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-γ receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-γ receptor is structurally related to the IL-10 receptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
Buch MH, Church LD, Churchman SM, Emery P, Geiler J, Horner EA, McDermott MF, Parmar R, Ponchel F
Clin. Exp. Rheumatol. 2012 30:534-42
Species: Human
Sample Type: Serum
Analysis of the Interferon Gamma (rs2430561, +874T/A) Functional Gene Variant in Relation to the Presence of Cardiovascular Events in Rheumatoid Arthritis.
Balsa A, Blanco R, Castaneda S, Corrales A, Fernandez-Gutierrez B, Garcia-Bermudez M, Gomez-Vaquero C, Gonzalez-Alvaro I, Gonzalez-Gay M, Gonzalez-Juanatey C, Llorca J, Lopez-Mejias R, Martin J, Miranda-Filloy J, Robledo G
PLoS ONE 2012 7:e47166
Species: Human
Sample Type: Serum
Potent adjuvanticity of a pure TLR7-agonistic imidazoquinoline dendrimer.
Balakrishna R, David S, Malladi S, Mutz C, Salunke D, Shukla N
PLoS ONE 2012 7:e43612
Species: Human
Sample Type: Cell Culture Supernates
Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
Abe Y, Akashi K, Daitoku S, Harada N, Iwasaki H, Kikushige Y, Kishimoto J, Kohno K, Kuriyama T, Miyamoto T, Takenaka K, Teshima T, Yamauchi T, Yoshimoto G
Blood 2012 120:4058-67
Species: Human
Sample Type: Serum
Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
Basta S, Hurlbut DJ, Justinich CJ, Mak N, Mulder DJ, Pooni A
Am. J. Pathol. 2011 178:744-53
Species: Human
Sample Type: Whole Tissue
HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
Anderson KC, Chauhan D, Exley MA, Hideshima T, Munshi NC, Nanjappa P, Song W, Tai YT, Tian Z
Leukemia 2011 25:161-8
Species: Human
Sample Type: Cell Culture Supernates
Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
Bieber T, Ferring-Schmidt S, Kummerer BM, Rehkamper C, Tuting T, Wenzel J, Zahn S
J. Invest. Dermatol. 2011 131:133-40
Species: Human
Sample Type: Cell Culture Supernates
Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties.
Brandau S, Bruderek K, Elian M, Lang S, Schenck M, Schmaltz D, Steller G, Suttmann H, Trellakis S, Welling J, Zabel P
J. Leukoc. Biol. 2011 89:311-7
Species: Human
Sample Type: Cell Culture Supernates
Subnormal cytokine profile in the tear fluid of keratoconus patients.
Chakravarti S, Cope L, Feng X, Hamad A, Jun AS, Lee S, Meng H, Speck C
PLoS ONE 2011 6:e16437
Species: Human
Sample Type: Tears
Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
Fu F, Gu W, Shen B, Shen Z, Sun J, Wang H, Yan R, Zhang G, Zhang X, Zhou Y
PLoS ONE 2011 6:e24751
Species: Human
Sample Type: Cell Culture Supernates
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