Human HGF Quantikine ELISA Kit | hHGF QKit

R&D Systems製のHuman HGFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。

The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Serum, EDTA Plasma, Citrate Plasma

The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Bieback K, Dietz L, Kinzebach S, Kluter H, Thierse H
BMC Cell Biol 2013 14:48
Species: Human
Sample Type: Cell Culture Supernates


The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
Jin P, Li Q, Li X, Tao C, Zhang A
Biochem Biophys Res Commun 2013 441:675-80
Species: Human
Sample Type: Cell Culture Supernates


The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells.
Balletshofer B, Dolderer JH, Haring HU, Konigsrainer A, Kuper M, Machann J, Machicao F, Meile T, Rittig K, Schaller HE, Schick F, Siegel-Axel DI, Staiger H, Stock UA
Diabetologia 2012 55:1514-25
Species: Human
Sample Type: Serum


Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Amadori D, Arvanitakis C, Badulescu F, Barrios C, Beaugrand M, Befeler A, Bennouna J, Blanc J-, Blum H, Bolondi L, Borbath I, Boyer M, Boyer T, Britten C, Bronowicki J-, Bruix J, Buggisch P, Burak K, Bustamante Schneider J, Byrne T, Caspary W, Cisnero Garza LE, Cognetti F, Cosme de Oliveira A, Craxi A, De Greve J, Degos F, Diago M, Dollinger M, Dominguez S, Falk S, Farinati F, Figer A, Forner A, Gamargo Garate C, Gane E, Garcia-Tsao G, Gerken G, Germanidis G, Goke B, Gold P, Goldenberg A, Grange J-, Gregor M, Greten T, Gridelli C, Haussinger D, Heuman D, Hilgard P, Hillon P, Jassem J, Jeffry Evans TR, Katsos I, Kennedy P, Koch A, Kotzev I, Kubyshkin V, Lathia CD, Leon J, Llovet JM, Lozano A, Ma M, Marrero J, Martinez-Castillo J, Martoni A, Mazzaferro V, Meinhardt G, Metrakos P, Meyer T, Miron L, Montero Alvarez JL, Muellhaupt B, O'Donnell A, Olweny C, Pallota MG, Pascual S, Pena CE, Porta C, Raoul J-, Reeves H, Ricci S, Riordan S, Ross P, Roth A, Ru??z del Arbol L, Rydzewska G, Sangiovanni A, Sangro B, Santoro A, Scher??bl J, Scheulen M, Schilsky M, Schmid R, Schwartz J, Schwartz M, Seitz J-, Shan M, Sherman M, Sol?? R, Spengler U, Stemmer S, Strickland A, Szawlowski A, Tabernero J, Takov D, Tchernev K, Tebbutt N, Thomson B, Tomczak P, Trevisani F, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Wiest R, Zarba JJ, Zeuzem S
Clin. Cancer Res. 2012 18:2290-300
Species: Human
Sample Type: Plasma


Validation and quality control of ELISAs for the use with human saliva samples.
Jaedicke KM, Preshaw PM, Taylor JJ
J. Immunol. Methods 2012 377:62-5

Comparison of the levels of the growth factors in umbilical cord serum and human milk and its clinical significance.
Bulbule A, Indumathi S, Kaingade P, Nikam A, Patil R, Patki S, Patki U, Pishte A
Cytokine 2012 59:305-8

Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor beta and Notch signaling.
Foraker JE, Prockop DJ, Robinson AP, Ylostalo J
Stem Cells Dev. 2011 20:289-300
Species: Human
Sample Type: Cell Culture Supernates


Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Cheng K, Li TS, Malliaras K, Marban E, Marban L, Matsushita N, Sun B, Zhang Y
Cardiovasc. Res. 2011 89:157-65
Species: Human
Sample Type: Cell Culture Supernates


Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
Asosingh K, Comhair S, Erzurum SC, Farha S, George D, Hsi E, Lichtin A, Loyd JE, Park M, Sharp J, Tang WH, Theil K, Tubbs R, Xu W
Blood 2011 117:3485-93
Species: Human
Sample Type: Plasma


Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Amann EM, Feifel E, Gstraunthaler G, Pfaller W, Rauch C, Schennach H, Spotl HP
ALTEX 2011 28:305-16
Species: Human
Sample Type: Cell Lysates