Human IGF-I Quantikine ELISA Kit | hIGF-I/IGF-1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111097
R&D Systems製のHuman IGF-Iを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月27日 00時00分現在]
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IGF-I/IGF-1, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年04月27日 00時00分現在]
IGF-I/IGF-1, Human, ELISA Kit, Quantikine (96well)
文献数： 87
| 説明文 |
Genbank No：
3479
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| 関連記事 |
R&D Systems社製品#DG100, #SG100, #PDG100の販売終了および改良版#DG100B, SG100B, PDG100Bの販売 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
- Sensitivity
- 0.056 ng/mL
- Assay Range
- 0.094 - 6 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human IGF-I
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.49 | 1.243 | 2.373 | 0.382 | 0.967 | 1.941 |
| Standard Deviation | 0.017 | 0.054 | 0.103 | 0.031 | 0.08 | 0.145 |
| CV% | 3.5 | 4.3 | 4.3 | 8.1 | 8.3 | 7.5 |
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Recovery
The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 99 | 97-102 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IGF-I
- Aliases
- IGF1; IGF-1; Somatomedin A; Somatomedin C
- Entrez Gene IDs
- 3479 (Human); 16000 (Mouse); 24482 (Rat)
- Background
- IGF Ligands
- Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conservedbetween the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Bieback K, Dietz L, Kinzebach S, Kluter H, Thierse H
BMC Cell Biol 2013 14:48
Species: Human
Sample Type: Cell Culture Supernates
Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
Brazil DP, Crossey PA, Higgins MF, McAuliffe FM, Nyhan KC, Russell NE
PLoS ONE 2012 7:e29164
Species: Human
Sample Type: Plasma
Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
Madry H, Venkatesan JK, Weimer A
Mol. Med. 2012 18:346-58
Species: Human
Sample Type: Cell Culture Supernates
Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
Currie MJ, Dachs GU, Robinson BA, Volkova E, Wells JE, Willis JA
Br. J. Cancer 2011 104:51-9
Species: Human
Sample Type: Serum
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Cheng K, Li TS, Malliaras K, Marban E, Marban L, Matsushita N, Sun B, Zhang Y
Cardiovasc. Res. 2011 89:157-65
Species: Human
Sample Type: Cell Culture Supernates
The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
Bilkey DK, Dennis NA, McLennan IS, Morgan K, Ruffman T
PLoS ONE 2011 6:e20533
Species: Human
Sample Type: Serum
Prognostic significance of angiogenic factors in uterine cervical cancer.
Blohmer JU, Heidecke H, Jeschke S, Korlach S, Kummel S, Landt S, Lichtenegger W, Schmid P, Sehouli J, Stoblen F, Wehling M
Anticancer Res. 2011 31:2589-95
Species: Human
Sample Type: Serum
Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
Berger AM, Bilek LD, Brusnahan SK, Garvin KL, Jackson JD, Kessinger MA, McGuire TR, O'Kane BJ, Sharp JG, Tuljapurkar SR
Obesity (Silver Spring) 2011 19:2130-6
Species: Human
Sample Type: Plasma
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Amann EM, Feifel E, Gstraunthaler G, Pfaller W, Rauch C, Schennach H, Spotl HP
ALTEX 2011 28:305-16
Species: Human
Sample Type: Cell Lysates
Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
Franco MC, Sesso R
Am. J. Hypertens. 2010 23:6-11
Species: Human
Sample Type: Plasma
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