Human CCL18/PARC Quantikine ELISA Kit | hPARC Qkit
掲載日情報：2016/08/18 現在Webページ番号：111057
R&D Systems製のHuman CCL18/PARCを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 10時55分現在]
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[在庫・価格 ：2025年04月29日 10時55分現在]
Human CCL18/PARC Quantikine ELISA Kit
文献数： 0
- 商品コード：DCL180
- メーカー：RSD
- 包装：1kit
- 本製品は取扱中止になりました
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Tissue Lysates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)
- Sensitivity
- 1.77 pg/mL
- Assay Range
- 18.8 - 1,200 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine, Tissue Lysates)
- Specificity
- Natural and recombinant human CCL18.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human CCL18/PARC Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human CCL18 in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains
E. coli-expressed recombinant human CCL18 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CCL18 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CCL18.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 70.8 | 248 | 592 | 84.2 | 265 | 614 |
| Standard Deviation | 2.27 | 8.21 | 21.8 | 5.1 | 17.1 | 27.6 |
| CV% | 3.2 | 3.3 | 3.7 | 6.1 | 6.5 | 4.5 |
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Recovery
The recovery of human CCL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 101 | 91-110 |
| Urine (n=4) | 104 | 92-112 |
| Tissue Lysis Buffer (n=1) | 92 | 91-93 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- CCL18/PARC
- Aliases
- AMAC-1; DC-CK1; MIP-4; PARC
- Entrez Gene IDs
- 6362 (Human)
- Background
- CCL18/PARC (Pulmonary and Activation Regulated Chemokine)
- Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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