Human TGF-beta 1 Quantikine ELISA Kit | hTGF-b1 QKit
掲載日情報：2017/09/01 現在Webページ番号：111021
R&D Systems製のHuman TGF-beta 1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 12時55分現在]
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TGF-β1, ELISA Kit, Quantikine, 2nd Generation (96well) |
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[在庫・価格 ：2025年04月26日 12時55分現在]
TGF-β1, ELISA Kit, Quantikine, 2nd Generation (96well)
文献数： 386
| 説明文 | Genbank No：
7040
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| 保存条件 | 法規備考 | ||
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies R&D Systems社製品#DB100Bの販売終了および改良版#DB100Cの販売 Quantikine TGF-β1 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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- ELISA kitトラブルシューティングガイド／FAQ
- Product Details
- Product Summary
- Precision
- Recovery
- Linearity
- Additional Infomation
- Citations
ELISA kitトラブルシューティングガイド／FAQ
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (40 µL), EDTA Plasma (40 µL), Urine (100 µL)
- Sensitivity
- 15.4 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant TGF-beta 1. This kit also recognizes human TGF-beta 1.2 and mouse, rat, porcine, and canine TGF-beta 1.
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human TGF-beta 1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TGF-beta 1 in acid activated cell culture supernates, serum, plasma, and urine. It contains recombinant human TGF-beta 1 expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural TGF-beta 1 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural human TGF-beta 1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 21 | 22 |
| Mean | 88 | 198 | 816 | 97 | 209 | 826 |
| Standard Deviation | 3 | 4.9 | 19.6 | 8.1 | 17.3 | 47 |
| CV% | 3.4 | 2.5 | 2.4 | 8.4 | 8.3 | 5.7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 28 | 37 | 30 |
| Mean | 78 | 180 | 695 | 79 | 185 | 730 |
| Standard Deviation | 2.3 | 3.4 | 17.3 | 7.2 | 17.2 | 46.8 |
| CV% | 2.9 | 1.9 | 2.5 | 9.1 | 9.3 | 6.4 |
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Recovery
The recovery of TGF-beta 1 spiked to levels throughout the range of the assay followed by activation was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 99 | 86-115 |
| Urine (n=3) | 96 | 88-100 |
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Linearity
To assess the linearity of the assay, activated samples containing and/or spiked with high concentrations of TGF-beta 1 were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- TGF-beta 1
- Aliases
- TGFB1
- Entrez Gene IDs
- 7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine)
- Background
- TGF-beta Family Ligands
- Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
Aoudjit F, Boisvert M, Chakir J, Fakhfakh R, Hadj-Salem I, Jacques E, Loubaki L, Plante S
PLoS ONE 2013 8:e81983
Species: Human
Sample Type: Cell Culture Supernates
Hypoxia potentiates glioma-mediated immunosuppression.
Fokt I, Fuller G, Heimberger AB, Kong LY, Melillo G, Priebe W, Wang Y, Wei J, Wu A
PLoS ONE 2012 6:e16195
Species: Human
Sample Type: Cell Culture Supernates
Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
Bouadma L, Crestani B, Dehoux M, Elbim C, Garnier M, Gelly J, Lasocki S, Lecon V, Mentre F, Nardelli L, Philip I, Piednoir P, Quesnel C, Soler P
Crit. Care Med. 2012 40:21-8
Species: Human
Sample Type: BALF
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Caillat-Zucman S, Choukem SP, Dupuy S, Lambert M, Lebbe C, Pages C, Tognarelli S, Zucman D
PLoS Pathog. 2012 8:e1002486
Species: Human
Sample Type: Cell Culture Supernates
Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.
Hsieh SC, Li KJ, Lu MC, Tsai CY, Wu CH, Yang CC, Yu CL
J. Biomed. Biotechnol. 2012 2012:759313
Species: Human
Sample Type: Urine
Altered circulating levels of matrix metalloproteinases and inhibitors associated with elevated type 2 cytokines in lymphatic filarial disease.
Anuradha R, Babu S, George JP, Kumaraswami V, Nutman TB, Pavankumar N
PLoS Negl Trop Dis 2012 6:e1681
Species: Human
Sample Type: Platelet-Poor Plasma
Central role of SREBP-2 in the pathogenesis of osteoarthritis.
Gkretsi V, Iliopoulos D, Kostopoulou F, Malizos KN, Oikonomou P, Poultsides L, Tsezou A
PLoS ONE 2012 7:e35753
Species: Human
Sample Type: Cell Culture Supernates
Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
Arcaro KF, Browne EP, Kliethermes B, Qin W, Ruhlen RL, Sauter ER, Zhang K
BMC Cancer 2012 12:100
Species: Human
Sample Type: Milk
Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
Buch MH, Church LD, Churchman SM, Emery P, Geiler J, Horner EA, McDermott MF, Parmar R, Ponchel F
Clin. Exp. Rheumatol. 2012 30:534-42
Species: Human
Sample Type: Serum
Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis.
Charbonneau M, Dubois CM, Harper K, Lauzier A, Paquette M
Arthritis Res. Ther. 2012 14:R159
Species: Human
Sample Type: Cell Culture Supernates
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